TY - JOUR
T1 - Cytoskeletal calmodulin-dependent protein kinase. Characterization, solubilization, and purification from rat brain
AU - Sahyoun, N.
AU - LeVine, H.
AU - Bronson, D.
AU - Siegel-Greenstein, F.
AU - Cuatrecasas, P.
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 1985
Y1 - 1985
N2 - Brain calmodulin-dependent protein kinase was isolated as the major cytoskeletal component of the postsynaptosomal fraction, accounting for about 50% of the total protein. The α- and β-subunits of the enzyme were present in a ratio of about 6:1. The cytoskeletal preparation was also distinguished by the regular presence of tubulin and actin in approximately equimolar amounts to the β-subunit. Electron microscopic morphology suggested that the enzyme formed a hierarchy of cytoskeletal complexes with an average diameter of 85 nm. These results indicate that cytoskeletal calmodulin-dependent protein kinase may have a structural as well as a catalytic role in neurons. The cytoskeletal enzyme could be solubilized in 8 M urea, displaying similar properties to its cytosolic counterpart. The postsynaptosomal cytoskeleton provides a simplified and well-defined model for the study of the protein-protein interactions involving calmodulin-dependent protein kinase.
AB - Brain calmodulin-dependent protein kinase was isolated as the major cytoskeletal component of the postsynaptosomal fraction, accounting for about 50% of the total protein. The α- and β-subunits of the enzyme were present in a ratio of about 6:1. The cytoskeletal preparation was also distinguished by the regular presence of tubulin and actin in approximately equimolar amounts to the β-subunit. Electron microscopic morphology suggested that the enzyme formed a hierarchy of cytoskeletal complexes with an average diameter of 85 nm. These results indicate that cytoskeletal calmodulin-dependent protein kinase may have a structural as well as a catalytic role in neurons. The cytoskeletal enzyme could be solubilized in 8 M urea, displaying similar properties to its cytosolic counterpart. The postsynaptosomal cytoskeleton provides a simplified and well-defined model for the study of the protein-protein interactions involving calmodulin-dependent protein kinase.
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M3 - Article
C2 - 4038501
AN - SCOPUS:0022006732
SN - 0021-9258
VL - 260
SP - 1230
EP - 1237
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 2
ER -