Cytotoxicity of dimethyl sulfoxide (DMSO) in direct contact with odontoblast-like cells

J. Hebling, L. Bianchi, F. G. Basso, D. L. Scheffel, D. G. Soares, M. R.O. Carrilho, D. H. Pashley, L. Tjäderhane, C. A. De Souza Costa

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


Objectives To evaluate the cytotoxicity of dimethyl sulfoxide (DMSO) on the repair-related activity of cultured odontoblast-like MDPC-23 cells. Methods Solutions with different concentrations of DMSO (0.05, 0.1, 0.3, 0.5 and 1.0 mM), diluted in culture medium (DMEM), were placed in contact with MDPC-23 cells (5 × 104 cells/cm2) for 24 h. Eight replicates (n = 8) were prepared for each solutions for the following methods of analysis: violet crystal dye for cell adhesion (CA), quantification of total protein (TP), alizarin red for mineralization nodules formation (MN) and cell death by necrosis (flow cytometry); while twelve replicates (n = 12) were prepared for viable cell number (Trypan Blue) and cell viability (MTT assay). Data were analyzed by ANOVA and Tukey or Kruskal-Wallis and Mann-Whitney's tests (p < 0.05). Results Cell viability, adhesion and percentage of cell death by necrosis were not affected by DMSO at any concentration, with no statistical significant difference among the groups. A significant reduction in total protein production was observed for 0.5 and 1.0 mM of DMSO compared to the control while increased mineralized nodules formation was seen only for 1.0 mM DMSO. Significance: DMSO caused no or minor cytotoxic effects on the pulp tissue repair-related activity of odontoblast-like cells.

Original languageEnglish
Pages (from-to)399-405
Number of pages7
JournalDental Materials
Issue number4
StatePublished - Apr 1 2015

Bibliographical note

Funding Information:
The present research was financially supported by the Brazilian Research Agencies São Paulo Research Foundation (FAPESP, 2012/08866-4 ) and National Counsel of Technological and Scientific Development (CNPq, grants 305204/2010-6 and 301291/2010-1 to JH, PI and grant 312768/2014-7 to MRC, PI), by the National Institute of Dental and Craniofacial Research ( R01 DE015306 to DHP, PI) and the Academy of Finland.

Publisher Copyright:
© 2015 Academy of Dental Materials.


  • Citotoxicity
  • Dimethyl sulfoxide
  • Odontoblast-like cells

ASJC Scopus subject areas

  • Materials Science (all)
  • Dentistry (all)
  • Mechanics of Materials


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