Daily rhythms in antennal protein and olfactory sensitivity in the malaria mosquito Anopheles gambiae

Samuel S.C. Rund, Nicolle A. Bonar, Matthew M. Champion, John P. Ghazi, Cameron M. Houk, Matthew T. Leming, Zainulabeuddin Syed, Giles E. Duffield

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

We recently characterized 24-hr daily rhythmic patterns of gene expression in Anopheles gambiae mosquitoes. These include numerous odorant binding proteins (OBPs), soluble odorant carrying proteins enriched in olfactory organs. Here we demonstrate that multiple rhythmically expressed genes including OBPs and takeout proteins, involved in regulating blood feeding behavior, have corresponding rhythmic protein levels as measured by quantitative proteomics. This includes AgamOBP1, previously shown as important to An. gambiae odorant sensing. Further, electrophysiological investigations demonstrate time-of-day specific differences in olfactory sensitivity of antennae to major host-derived odorants. The pre-dusk/dusk peaks in OBPs and takeout gene expression correspond with peak protein abundance at night, and in turn coincide with the time of increased olfactory sensitivity to odorants requiring OBPs and times of increased blood-feeding behavior. This suggests an important role for OBPs inmodulating temporal changes in odorant sensitivity, enabling the olfactory system to coordinate with the circadian niche of An. gambiae.

Original languageEnglish
Article number2494
JournalScientific Reports
Volume3
DOIs
StatePublished - Aug 29 2013

Bibliographical note

Funding Information:
This research was supported by grants (to G.E.D.) from Eck Institute for Global Health, and the Center for Rare and Neglected Diseases, University of Notre Dame (UND); and the Indiana Clinical Translational Sciences Institute; and UND College of Science-Summer Undergraduate Research Fellowships (to J.P.G. and C.M.H.). We thank L. Zwiebel and R. Pitts for anti-Agam\Orco serum54; R. Hut for providing CircWave v1.4 software; X. Li for technical assistance; and B. Boggess and M. Joyce for proteomics assistance. OBP crystal structure cartoons in Fig. 1 are from15 and Protein Data Bank (PDB) 3R1O. Mosquito head image from USDA Technical Bulletin 1447.

ASJC Scopus subject areas

  • General

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