DARPP-32 binds to tra2-beta1 and influences alternative splicing

Natalya Benderska, Kristina Becker, Jean Antoine Girault, Cord Michael Becker, Athena Andreadis, Stefan Stamm

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The majority of human genes undergo alternative splicing, which is frequently altered in response to physiological stimuli. DARPP-32 (dopamine and cAMP regulated phosphoprotein, 32 kDa) is a component of PKA-dependent signaling pathways. Here we show that DARPP-32 binds directly to the splicing factor tra2- beta1 (transformer 2). DARPP-32 changes the usage of tra2-beta1 dependent alternative exons in a concentration-dependent manner, suggesting that the DARPP-32:tra2-beta1 interaction is a molecular link between signaling pathways and pre-mRNA processing.

Original languageEnglish
Pages (from-to)448-453
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1799
Issue number5-6
DOIs
StatePublished - 2010

Keywords

  • Alternative splicing
  • DARPP-32
  • Protein phosphatase 1
  • RNA processing
  • Signaling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics

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