Data for high-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins

Yiran Feng; Xiaolan Yang; Huimin Chong; Deqiang Wang; Xiaolei Hu; Chang Guo Zhan; Fei Liao

doi:10.1016/j.dib.2017.07.019

Data for high-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins

Yiran Feng, Xiaolan Yang, Huimin Chong, Deqiang Wang, Xiaolei Hu, Chang Guo Zhan, Fei Liao

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Abstract

Data in this article are associated with the research article “Highthroughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins” (Yang et al., 2017) [1]. This article provided data on how to develop an immunoturbidimetric assay (ITA) of enzyme/mutants as proteins in cell lysates in high-throughput (HTP) mode together with HTP assay of their activities to derive their specific activities in cell lysates for comparison, with Pseudomonas aeruginosa arylsulfatase (PAAS) and Bacillus fastidious uricase (BFU) plus their mutants as models. Data were made publicly available for further analyses.

Original language	English
Pages (from-to)	220-245
Number of pages	26
Journal	Data in Brief
Volume	14
DOIs	https://doi.org/10.1016/j.dib.2017.07.019
State	Published - Oct 2017

Bibliographical note

Publisher Copyright:
© 2017 The Authors

Funding

This work was supported in part by the National Natural Science Foundation of China, China (Nos. 31570862, and 21402108), and the Ministry of Education of China (No. 20125503110007).

Funders	Funder number
National Natural Science Foundation of China (NSFC)	21402108, 31570862
Ministry of Education of the People's Republic of China	20125503110007

Keywords

HTP assay
Immunoturbidimetric assay
Mutant
Screen
Specific activity

ASJC Scopus subject areas

General

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10.1016/j.dib.2017.07.019

Cite this

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title = "Data for high-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins",

abstract = "Data in this article are associated with the research article “Highthroughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins” (Yang et al., 2017) [1]. This article provided data on how to develop an immunoturbidimetric assay (ITA) of enzyme/mutants as proteins in cell lysates in high-throughput (HTP) mode together with HTP assay of their activities to derive their specific activities in cell lysates for comparison, with Pseudomonas aeruginosa arylsulfatase (PAAS) and Bacillus fastidious uricase (BFU) plus their mutants as models. Data were made publicly available for further analyses.",

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AU - Feng, Yiran

AU - Yang, Xiaolan

AU - Chong, Huimin

AU - Wang, Deqiang

AU - Hu, Xiaolei

AU - Zhan, Chang Guo

AU - Liao, Fei

PY - 2017/10

Y1 - 2017/10

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AB - Data in this article are associated with the research article “Highthroughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins” (Yang et al., 2017) [1]. This article provided data on how to develop an immunoturbidimetric assay (ITA) of enzyme/mutants as proteins in cell lysates in high-throughput (HTP) mode together with HTP assay of their activities to derive their specific activities in cell lysates for comparison, with Pseudomonas aeruginosa arylsulfatase (PAAS) and Bacillus fastidious uricase (BFU) plus their mutants as models. Data were made publicly available for further analyses.

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KW - Immunoturbidimetric assay

KW - Mutant

KW - Screen

KW - Specific activity

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Data for high-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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