TY - JOUR
T1 - Decidual prolactin (prl)-releasing factor stimulates the synthesis of prl from human decidual cells
AU - Golander, A.
AU - Richards, R.
AU - Thrailkill, K.
AU - Capel, D.
AU - Rogers, D.
AU - Handwerger, S.
PY - 1988/7/1
Y1 - 1988/7/1
N2 - Previous studies from our laboratory demonstrated that the acute release of PRL from human decidual tissue is stimulated by a 23.5 kilodalton placental protein which we designated decidual PRL-releasing factor (PRL-RF). To determine whether PRL-RF may also affect the synthesis of PRL and/or cause a secondary increase in PRL release, we have examined the effects of purified PRL-RF on the synthesis and release of PRL over a 96-h period. Exposure of dispersed decidual cells to PRL-RF (0.5µg/nil) stimulated a biphasic increase in PRL release with acute transient stimulation during the first 0.5 h and a delayed and sustained stimulation beginning about 8 h after exposure which persisted for the duration of the 96 h. The amounts of PRL released from PRL-RF-exposed cells after 0.5, 8, 12, 24, and 96 h were 321.2 ± 36.2 (mean ± SEM, n = 3), 110.2 ± 5.3,138.2 ± 7.2± 11.2, and 201.5 ± 14.2% that of control cells. Studies of the de novo synthesis of [35S]methionyl PRL indicated that the increase in PRL release after the first few hours of exposure to PRL-RF was secondary to an increase in PRL synthesis. Somatostatin (100 nM) inhibited the acute stimulatory effect of PRL-RF, but had no effect on the delayed stimulation of PRL release. On the other hand, cycloheximide (20 Î�M) completely inhibited the secondary increase in PRL release in response to PRL-RF but had no effect on the acute release. These results demonstrate that PRL-RF stimulates both the synthesis and release of decidual PRL.
AB - Previous studies from our laboratory demonstrated that the acute release of PRL from human decidual tissue is stimulated by a 23.5 kilodalton placental protein which we designated decidual PRL-releasing factor (PRL-RF). To determine whether PRL-RF may also affect the synthesis of PRL and/or cause a secondary increase in PRL release, we have examined the effects of purified PRL-RF on the synthesis and release of PRL over a 96-h period. Exposure of dispersed decidual cells to PRL-RF (0.5µg/nil) stimulated a biphasic increase in PRL release with acute transient stimulation during the first 0.5 h and a delayed and sustained stimulation beginning about 8 h after exposure which persisted for the duration of the 96 h. The amounts of PRL released from PRL-RF-exposed cells after 0.5, 8, 12, 24, and 96 h were 321.2 ± 36.2 (mean ± SEM, n = 3), 110.2 ± 5.3,138.2 ± 7.2± 11.2, and 201.5 ± 14.2% that of control cells. Studies of the de novo synthesis of [35S]methionyl PRL indicated that the increase in PRL release after the first few hours of exposure to PRL-RF was secondary to an increase in PRL synthesis. Somatostatin (100 nM) inhibited the acute stimulatory effect of PRL-RF, but had no effect on the delayed stimulation of PRL release. On the other hand, cycloheximide (20 Î�M) completely inhibited the secondary increase in PRL release in response to PRL-RF but had no effect on the acute release. These results demonstrate that PRL-RF stimulates both the synthesis and release of decidual PRL.
UR - http://www.scopus.com/inward/record.url?scp=0023684563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023684563&partnerID=8YFLogxK
U2 - 10.1210/endo-123-1-335
DO - 10.1210/endo-123-1-335
M3 - Article
C2 - 2898361
AN - SCOPUS:0023684563
SN - 0013-7227
VL - 123
SP - 335
EP - 339
JO - Endocrinology
JF - Endocrinology
IS - 1
ER -