Deferoxamine inhibition of Cr(V)-mediated radical generation and deoxyguanine hydroxylation: ESR and HPLC evidence

Xianglin Shi, Xiaoya Sun, Peter M. Gannett, N. S. Dalal

Research output: Contribution to journalArticlepeer-review

74 Scopus citations


Electron spin resonance (ESR) and high-performance liquid chromatography (HPLC) techniques were utilized to investigate the effect of deferoxamine on free radical generation in the reaction of Cr(V) with H2O2 and organic hydroperoxides. ESR measurements demonstrated that deferoxamine can efficiently reduce the concentration of the Cr(V) intermediate as formed in the reduction of Cr(VI) by NAD(P)H or a flavoenzyme glutathione reductase/NADH. ESR spin trapping studies showed that deferoxamine also inhibits Cr(V)-mediated ·OH radical generation from H2O2, as well as Cr(V)-mediated alkyl and alkoxy radical formation from t-butyl hydroperoxide and cumene hydroperoxide. HPLC measurements showed that ·OH radicals generated by the Cr(VI)/flavoenzyme/ NAD(P)H enzymatic system react with 2′-deoxyguanine to form 8-hydroxy-2′-deoxyguanine (8-OHdG), a DNA damage marker. Deferoxamine effectly inhibited the formation of 8-OHdG also.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalArchives of Biochemistry and Biophysics
Issue number2
StatePublished - Mar 1992

Bibliographical note

Funding Information:
’ This research has been supported by the Department of the Interior’s Mineral Institute program administered by the Bureau of Mines through the Generic Mineral Technology Center for Respirable Dust under Grant G1135142 and by NIOSH Grant U60-CCU306149-01-1. ’ To whom correspondence and requests for reprints should be addressed.

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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