Delivery of short hairpin RNA in the neotropical brown stink bug, Euschistus heros, using a composite nanomaterial

Jérôme Laisney, Vanessa Loczenski Rose, Kayla Watters, Kevin V. Donohue, Jason M. Unrine

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The response of insects to orally delivered double-stranded RNA ranges widely among taxa studied to date. Long dsRNA does elicit a response in stink bugs but the dose required to achieve an effect is relatively high compared to other insects such Colorado potato beetle or western corn rootworm. Improving the delivery of dsRNA to stink bugs will improve the likelihood of using RNA-based biocontrols for the management of these economically important pests. Short hairpin RNA (shRNA) is a useful molecule with which to test improvements in the delivery of double stranded RNA in the neotropical brown stink bug, Euschistus heros, since shRNA alone does not elicit a clear effect like that for long dsRNA. Here, we show for the first time the oral delivery of shRNA triggering RNA interference (RNAi) in E. heros using 4 nm cerium oxide nanoparticles (CeO2 NPs) coated with diethylamioethyl dextran (Dextran-DEAE) as a carrier. We identified particle properties (coating composition and degree of substitution, hydrodynamic diameter, and zeta potential) and shRNA loading rates (Ce:shRNA mass ratio) that resulted in successful transcript reduction or RNAi. When the Z-average diameter of CeO2 Dextran-DEAE-shRNA NP complex was less than 250 nm and the zeta potential was in the 15–25 mV range (Ce:shRNA mass ratio of 0.7:1), significant mortality attributed to RNAi was observed with a shRNA concentration in feeding solution of 250 ng/μl. The degradation of the targeted troponin transcript by NP-delivered shRNA was equivalent to that observed with long dsRNA, while naked shRNA transcript reduction was not statistically significant. Elemental mapping by synchrotron X-ray fluorescence microprobe confirmed uptake and distribution of Ce throughout the body with the highest concentrations found in gut tissue. Taken together, our results suggest that a nanoparticle delivery system can improve the delivery of RNA-based biocontrols to E. heros, and therefore its attractiveness as an application in the management of this important pest in soybean production.

Original languageEnglish
Article number104906
JournalPesticide Biochemistry and Physiology
Volume177
DOIs
StatePublished - Aug 2021

Bibliographical note

Funding Information:
This research was supported by Syngenta Crop Protection , LLC through contract # 8600152814 with the University of Kentucky Research Foundation . Parts of this research used the XFM Beamline of the National Synchrotron Light Source II, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Brookhaven National Laboratory under Contract No. DE-SC0012704. The authors gratefully acknowledge R. Palli, R. Tappero, J. Li, Z. Elhaj Baddar and Y. Naudet.

Funding Information:
This research was supported by Syngenta Crop Protection, LLC through contract # 8600152814 with the University of Kentucky Research Foundation. Parts of this research used the XFM Beamline of the National Synchrotron Light Source II, a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Brookhaven National Laboratory under Contract No. DE-SC0012704. The authors gratefully acknowledge R. Palli, R. Tappero, J. Li, Z. Elhaj Baddar and Y. Naudet.

Publisher Copyright:
© 2021 Elsevier Inc.

Keywords

  • Cerium oxide
  • Delivery
  • Insect
  • Nanocomposite
  • RNAi
  • Short hairpin RNA

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

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