Demonstration and nature of endothelin-3-like immunoreactivity in somatostatin and choline acetyltransferase-immunoreactive nerve cells of the neostriatum of the rat

K. Fuxe, B. Tinner, W. Staines, A. Hemsén, L. Hersh, J. M. Lundberg

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Using a rabbit endothelin-1 (ET-1) antiserum together with a goat-anticholineacetylase antiserum or a mouse anti-somatostatin antiserum it was possible by means of double immunolabelling procedures to demonstrate ET-like immunoreactivity in striatal nerve cell bodies of the rat, which were shown to contain either cholineacetylase or somatostatin immunoreactivity. Absorption studies with ET-3, ET-1 or big ET-1 indicated that the ET-like immunoreactivity was ET-3 like. In agreement the radioimmunoassay showed that ET-3-like immunoreactivity was present in higher concentrations that ET-1-like immunoreactivity in the neostriatum and other brain areas. Characterization by reversed phase HPLC revealed, however, that a major portion of the neostriatal ET-3-like immunoreactivity was not identical to ET-3. The nature of neuronal ET in the rat may thus be more complicated than hitherto assumed.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalNeuroscience Letters
Volume123
Issue number1
DOIs
StatePublished - Feb 11 1991

Bibliographical note

Funding Information:
This work has been supported by the Swedish Medical Research Council (04X-715; 14X-6554) and the American Council for Tobacco Research.

Keywords

  • Cholineacetylase
  • Endothelin-3
  • Immunocytochemistry
  • Neostriatum
  • Nerve cell
  • Radioimmunoassay
  • Rat
  • Somatostatin

ASJC Scopus subject areas

  • Neuroscience (all)

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