Dendritic Cell Membrane-Derived Nanovesicles for Targeted T Cell Activation

Brock T. Harvey, Xu Fu, Lan Li, Khaga R. Neupane, Namrata Anand, Jill M. Kolesar, Christopher I. Richards

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


T cells play an integral role in the generation of an effective immune response and are responsible for clearing foreign microbes that have bypassed innate immune system defenses and possess cognate antigens. The immune response can be directed toward a desired target through the selective priming and activation of T cells. Due to their ability to activate a T cell response, dendritic cells and endogenous vesicles from dendritic cells are being developed for cancer immunotherapy treatment. However, current platforms, such as exosomes and synthetic nanoparticles, are limited by their production methods and application constraints. Here, we engineer nanovesicles derived from dendritic cell membranes with similar properties as dendritic cell exosomes via nitrogen cavitation. These cell-derived nanovesicles are capable of activating antigen-specific T cells through direct and indirect mechanisms. Additionally, these nanovesicles can be produced in large yields, overcoming production constraints that limit clinical application of alternative immunomodulatory vesicle or nanoparticle-based methods. Thus, dendritic cell-derived nanovesicles generated by nitrogen cavitation show potential as an immunotherapy platform to stimulate and direct T cell response.

Original languageEnglish
Pages (from-to)46222-46233
Number of pages12
JournalACS Omega
Issue number50
StatePublished - Dec 20 2022

Bibliographical note

Funding Information:
Support for this work was provided by the Kentucky Pediatric Cancer Research Trust Fund (PON2 728 2000002500).

Funding Information:
We thank the Flow Cytometry Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558) and Light Microscopy Core at the University of Kentucky for flow cytometry and confocal microscopy experiments, respectively. Access to characterization instruments and staff assistance was provided by the Electron Microscopy Center at the University of Kentucky, member of the KY INBR (Kentucky IDeA Networks for Biomedical Research Excellence), which is funded by the National Institute of Health (NIH) National Institute of General Medical Science (IDeA Grant P20GM103436), and of the National Nanotechnology Coordinated Infrastructure (NNCI), which is supported by the National Science Foundation (ECCS-1542164). Illustrations were created with .

Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.

ASJC Scopus subject areas

  • Chemistry (all)
  • Chemical Engineering (all)


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