Abstract
To date, studies that have aimed to investigate the role of satellite cells during adult skeletal muscle adaptation and hypertrophy have utilized a nontranslational stimulus and/or have been performed over a relatively short time frame. Although it has been shown that satellite cell depletion throughout adulthood does not drive skeletal muscle loss in sedentary mice, it remains unknown how satellite cells participate in skeletal muscle adaptation to long-term physical activity. The current study was designed to determine whether reduced satellite cell content throughout adulthood would influence the transcriptome-wide response to physical activity and diminish the adaptive response of skeletal muscle. We administered vehicle or tamoxifen to adult Pax7-diphtheria toxin A (DTA) mice to deplete satellite cells and assigned them to sedentary or wheel-running conditions for 13 mo. Satellite cell depletion throughout adulthood reduced balance and coordination, overall running volume, and the size of muscle proprioceptors (spindle fibers). Furthermore, satellite cell participation was necessary for optimal muscle fiber hypertrophy but not adaptations in fiber type distribution in response to lifelong physical activity. Transcriptome-wide analysis of the plantaris and soleus revealed that satellite cell function is muscle type specific; satellite cell-dependent myonuclear accretion was apparent in oxidative muscles, whereas initiation of G protein-coupled receptor (GPCR) signaling in the glycolytic plantaris may require satellite cells to induce optimal adaptations to long-term physical activity. These findings suggest that satellite cells play a role in preserving physical function during aging and influence muscle adaptation during sustained periods of physical activity.
Original language | English |
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Pages (from-to) | C1178-C1188 |
Journal | American Journal of Physiology - Cell Physiology |
Volume | 318 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2020 |
Bibliographical note
Publisher Copyright:Copyright © 2020 the American Physiological Society.
Funding
This work was supported by National Institutes of Health (NIH) Grants AG-060701 and AG-049806 to C. A. Peterson and J. J. McCarthy and Grant AR-071753 to K. A. Murach. The project described was also supported by the NIH National Center for Advancing Translational Sciences through Grant TL1-TR-001997 (D. A. Englund).
Funders | Funder number |
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National Institutes of Health (NIH) | AG-060701, AG-049806 |
National Institute of Arthritis and Musculoskeletal and Skin Diseases | F32AR071753 |
National Center for Advancing Translational Sciences (NCATS) | TL1-TR-001997 |
Keywords
- Aging
- Exercise
- Satellite cells
- Skeletal muscle
- Stem cells
ASJC Scopus subject areas
- Physiology
- Cell Biology