TY - JOUR
T1 - Design and construction of a versatile system for the expression of foreign genes in plants
AU - Schardl, Christopher L.
AU - Byrd, Alfred D.
AU - Benzion, Gary
AU - Altschuler, Mitchell A.
AU - Hildebrand, David F.
AU - Hunt, Arthur G.
PY - 1987
Y1 - 1987
N2 - We have built a series of vectors to allow the constitutive or light-regulated expression of foreign genes in plants. These vectors carry expression cassettes consisting of either the cauliflower mosaic virus 35S promoter or the pea rbcS-E9 promoter, a multiple cloning site derived from M13um20, and the rbcS-E9 polyadenylation site. These cassettes have been incorporated into pBR322-based or RK2-based replicons to facilitate direct DNA uptake or Agrobacterium tumefaciens-mediated gene transfer. Their application for the expression of a bacterial gene is described.
AB - We have built a series of vectors to allow the constitutive or light-regulated expression of foreign genes in plants. These vectors carry expression cassettes consisting of either the cauliflower mosaic virus 35S promoter or the pea rbcS-E9 promoter, a multiple cloning site derived from M13um20, and the rbcS-E9 polyadenylation site. These cassettes have been incorporated into pBR322-based or RK2-based replicons to facilitate direct DNA uptake or Agrobacterium tumefaciens-mediated gene transfer. Their application for the expression of a bacterial gene is described.
KW - Agrobacterium tumefaciens
KW - Recombinant DNA
KW - cauliflower mosaic virus
KW - expression vectors
KW - light-regulated vectors
KW - pea promoter
KW - plant transformation
KW - polyadenylation site
KW - transfection
UR - http://www.scopus.com/inward/record.url?scp=0023575311&partnerID=8YFLogxK
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U2 - 10.1016/0378-1119(87)90359-3
DO - 10.1016/0378-1119(87)90359-3
M3 - Article
C2 - 3443303
AN - SCOPUS:0023575311
SN - 0378-1119
VL - 61
SP - 1
EP - 11
JO - Gene
JF - Gene
IS - 1
ER -