Detailed mapping of the linear B Cell epitopes of the hemagglutinin (HA) protein of swine influenza virus

Zhao Wang, Bing Huang, Milton Thomas, Chithra C. Sreenivasan, Zizhang Sheng, Jieshi Yu, Ben M. Hause, Dan Wang, David H. Francis, Radhey S. Kaushik, Feng Li

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Using the Hemagglutinin (HA) protein peptide array of H1N1 pdm09 and a panel of swine antisera against various swine influenza H1 and H3 clusters, we identified three immunoreactive epitopes with one (peptide 15) located in HA1 (amino acids 57–71) and two (peptides 121 and 139) in HA2 (amino acids 481–495 and 553–566). Further analysis showed that all swine antisera of H1 clusters efficiently recognized two HA2 epitopes; peptides 121 and 139, with only a subset of antisera reactive to HA1-derived peptide 15. Interestingly, none of these peptides were reactive to SIV H3 antisera. Finally, intranasal inoculation of peptides 15 and 121 into pigs revealed that peptide 121, not peptide 15, was able to generate antibody responses in some animals. The results of our experiments provide an important foundation for further analyzing the immune response against these peptides during natural viral infection and also provide peptide substrates for diagnostic assays.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
StatePublished - Sep 2018

Bibliographical note

Funding Information:
This work was partially supported by SDSU AES 3AH-477, by National Science Foundation/EPSCoR ( ) award IIA-1335423 , and by the state of South Dakota Governor's Office of Economic Development as a South Dakota Research Innovation Center. Radhey S. Kaushik was funded and supported by USDA NIFA SDSU Agricultural Experiment Station Hatch grant # SD00H547-15 .

Publisher Copyright:
© 2018 Elsevier Inc.


  • B cell epitope
  • H1 subtype
  • H3 subtype
  • Hemagglutinin (HA) Protein
  • Peptide
  • Swine influenza

ASJC Scopus subject areas

  • Virology


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