Detailed mapping of the linear B Cell epitopes of the hemagglutinin (HA) protein of swine influenza virus

Zhao Wang; Bing Huang; Milton Thomas; Chithra C. Sreenivasan; Zizhang Sheng; Jieshi Yu; Ben M. Hause; Dan Wang; David H. Francis; Radhey S. Kaushik; Feng Li

doi:10.1016/j.virol.2018.07.013

Detailed mapping of the linear B Cell epitopes of the hemagglutinin (HA) protein of swine influenza virus

Zhao Wang, Bing Huang, Milton Thomas, Chithra C. Sreenivasan, Zizhang Sheng, Jieshi Yu, Ben M. Hause, Dan Wang, David H. Francis, Radhey S. Kaushik, Feng Li

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Using the Hemagglutinin (HA) protein peptide array of H1N1 pdm09 and a panel of swine antisera against various swine influenza H1 and H3 clusters, we identified three immunoreactive epitopes with one (peptide 15) located in HA1 (amino acids 57–71) and two (peptides 121 and 139) in HA2 (amino acids 481–495 and 553–566). Further analysis showed that all swine antisera of H1 clusters efficiently recognized two HA2 epitopes; peptides 121 and 139, with only a subset of antisera reactive to HA1-derived peptide 15. Interestingly, none of these peptides were reactive to SIV H3 antisera. Finally, intranasal inoculation of peptides 15 and 121 into pigs revealed that peptide 121, not peptide 15, was able to generate antibody responses in some animals. The results of our experiments provide an important foundation for further analyzing the immune response against these peptides during natural viral infection and also provide peptide substrates for diagnostic assays.

Original language	English
Pages (from-to)	131-137
Number of pages	7
Journal	Virology
Volume	522
DOIs	https://doi.org/10.1016/j.virol.2018.07.013
State	Published - Sep 2018

Bibliographical note

Publisher Copyright:
© 2018 Elsevier Inc.

Funding

This work was partially supported by SDSU AES 3AH-477, by National Science Foundation/EPSCoR ( http://www.nsf.gov/od/iia/programs/epscor/index.jsp ) award IIA-1335423 , and by the state of South Dakota Governor's Office of Economic Development as a South Dakota Research Innovation Center. Radhey S. Kaushik was funded and supported by USDA NIFA SDSU Agricultural Experiment Station Hatch grant # SD00H547-15 .

Funders	Funder number
South Dakota Governor's Office of Economic Development
USDA NIFA SDSU Agricultural Experiment Station Hatch	SD00H547-15
National Science Foundation Arctic Social Science Program
National Institute of Allergy and Infectious Diseases	R15AI089690
National Institute of Allergy and Infectious Diseases
Office of Experimental Program to Stimulate Competitive Research	IIA-1335423
Office of Experimental Program to Stimulate Competitive Research
San Diego State University	AES 3AH-477
San Diego State University

Keywords

B cell epitope
H1 subtype
H3 subtype
Hemagglutinin (HA) Protein
Peptide
Swine influenza

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2018.07.013

Cite this

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title = "Detailed mapping of the linear B Cell epitopes of the hemagglutinin (HA) protein of swine influenza virus",

abstract = "Using the Hemagglutinin (HA) protein peptide array of H1N1 pdm09 and a panel of swine antisera against various swine influenza H1 and H3 clusters, we identified three immunoreactive epitopes with one (peptide 15) located in HA1 (amino acids 57–71) and two (peptides 121 and 139) in HA2 (amino acids 481–495 and 553–566). Further analysis showed that all swine antisera of H1 clusters efficiently recognized two HA2 epitopes; peptides 121 and 139, with only a subset of antisera reactive to HA1-derived peptide 15. Interestingly, none of these peptides were reactive to SIV H3 antisera. Finally, intranasal inoculation of peptides 15 and 121 into pigs revealed that peptide 121, not peptide 15, was able to generate antibody responses in some animals. The results of our experiments provide an important foundation for further analyzing the immune response against these peptides during natural viral infection and also provide peptide substrates for diagnostic assays.",

keywords = "B cell epitope, H1 subtype, H3 subtype, Hemagglutinin (HA) Protein, Peptide, Swine influenza",

author = "Zhao Wang and Bing Huang and Milton Thomas and Sreenivasan, \{Chithra C.\} and Zizhang Sheng and Jieshi Yu and Hause, \{Ben M.\} and Dan Wang and Francis, \{David H.\} and Kaushik, \{Radhey S.\} and Feng Li",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

year = "2018",

month = sep,

doi = "10.1016/j.virol.2018.07.013",

language = "English",

volume = "522",

pages = "131--137",

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T1 - Detailed mapping of the linear B Cell epitopes of the hemagglutinin (HA) protein of swine influenza virus

AU - Wang, Zhao

AU - Huang, Bing

AU - Thomas, Milton

AU - Sreenivasan, Chithra C.

AU - Sheng, Zizhang

AU - Yu, Jieshi

AU - Hause, Ben M.

AU - Wang, Dan

AU - Francis, David H.

AU - Kaushik, Radhey S.

AU - Li, Feng

PY - 2018/9

Y1 - 2018/9

N2 - Using the Hemagglutinin (HA) protein peptide array of H1N1 pdm09 and a panel of swine antisera against various swine influenza H1 and H3 clusters, we identified three immunoreactive epitopes with one (peptide 15) located in HA1 (amino acids 57–71) and two (peptides 121 and 139) in HA2 (amino acids 481–495 and 553–566). Further analysis showed that all swine antisera of H1 clusters efficiently recognized two HA2 epitopes; peptides 121 and 139, with only a subset of antisera reactive to HA1-derived peptide 15. Interestingly, none of these peptides were reactive to SIV H3 antisera. Finally, intranasal inoculation of peptides 15 and 121 into pigs revealed that peptide 121, not peptide 15, was able to generate antibody responses in some animals. The results of our experiments provide an important foundation for further analyzing the immune response against these peptides during natural viral infection and also provide peptide substrates for diagnostic assays.

AB - Using the Hemagglutinin (HA) protein peptide array of H1N1 pdm09 and a panel of swine antisera against various swine influenza H1 and H3 clusters, we identified three immunoreactive epitopes with one (peptide 15) located in HA1 (amino acids 57–71) and two (peptides 121 and 139) in HA2 (amino acids 481–495 and 553–566). Further analysis showed that all swine antisera of H1 clusters efficiently recognized two HA2 epitopes; peptides 121 and 139, with only a subset of antisera reactive to HA1-derived peptide 15. Interestingly, none of these peptides were reactive to SIV H3 antisera. Finally, intranasal inoculation of peptides 15 and 121 into pigs revealed that peptide 121, not peptide 15, was able to generate antibody responses in some animals. The results of our experiments provide an important foundation for further analyzing the immune response against these peptides during natural viral infection and also provide peptide substrates for diagnostic assays.

KW - B cell epitope

KW - H1 subtype

KW - H3 subtype

KW - Hemagglutinin (HA) Protein

KW - Peptide

KW - Swine influenza

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SN - 0042-6822

VL - 522

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JF - Virology

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Detailed mapping of the linear B Cell epitopes of the hemagglutinin (HA) protein of swine influenza virus

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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