Detection and survey of coffee ringspot virus in Brazil

T. O. Ramalho; A. R. Figueira; R. Wang; O. Jones; L. E. Harris; M. M. Goodin

doi:10.1007/s00705-015-2663-0

Detection and survey of coffee ringspot virus in Brazil

T. O. Ramalho, A. R. Figueira, R. Wang, O. Jones, L. E. Harris, M. M. Goodin

Plant Pathology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Coffee ringspot virus (CoRSV) a member of the proposed genus “Dichorhavirus”, was surveyed on commercial and research farms spanning an area responsible for the majority of Coffea arabica production in Brazil. Virus-infected plants were found at one hundred percent of locations (n = 45) sampled. All cultivars, regardless of cherry color, were found to serve as hosts, suggesting that there is limited resistance in commercially employed germplasm. Reverse transcription PCR analysis revealed that the virus is contained within symptomatic lesions, with little systemic spread throughout leaves. Phylogenetic analysis based on the ORF1 (nucleocapsid) gene identified a strong geo-spatial relationship among isolates, which clustered into three clades. Despite low genetic diversity among isolates, variation in symptom expression was observed in the experimental host Chenopodium quinoa. Our analyses support the hypothesis that the spread of CoRSV is constrained by the clonal expansion of thelytokous populations of Brevipalpus phoenicis. The widespread occurrence of this virus suggests that it is much more prevalent than previously thought.

Original language	English
Pages (from-to)	335-343
Number of pages	9
Journal	Archives of Virology
Volume	161
Issue number	2
DOIs	https://doi.org/10.1007/s00705-015-2663-0
State	Published - Feb 1 2016

Bibliographical note

Funding Information:
We thank the CNPq, CAPES, FAPEMIG, and NSF funding agencies for providing research grants to M.G. and A.F. We would also like to thank the farmers and cooperative workers who graciously provided access to their farms in support of this research. This publication recognizes CAPES Project No. A009, 1901133, and NSF-IOS-0749519).

Publisher Copyright:
© 2015, Springer-Verlag Wien.

ASJC Scopus subject areas

Virology

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10.1007/s00705-015-2663-0

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title = "Detection and survey of coffee ringspot virus in Brazil",

abstract = "Coffee ringspot virus (CoRSV) a member of the proposed genus “Dichorhavirus”, was surveyed on commercial and research farms spanning an area responsible for the majority of Coffea arabica production in Brazil. Virus-infected plants were found at one hundred percent of locations (n = 45) sampled. All cultivars, regardless of cherry color, were found to serve as hosts, suggesting that there is limited resistance in commercially employed germplasm. Reverse transcription PCR analysis revealed that the virus is contained within symptomatic lesions, with little systemic spread throughout leaves. Phylogenetic analysis based on the ORF1 (nucleocapsid) gene identified a strong geo-spatial relationship among isolates, which clustered into three clades. Despite low genetic diversity among isolates, variation in symptom expression was observed in the experimental host Chenopodium quinoa. Our analyses support the hypothesis that the spread of CoRSV is constrained by the clonal expansion of thelytokous populations of Brevipalpus phoenicis. The widespread occurrence of this virus suggests that it is much more prevalent than previously thought.",

author = "Ramalho, {T. O.} and Figueira, {A. R.} and R. Wang and O. Jones and Harris, {L. E.} and Goodin, {M. M.}",

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AU - Jones, O.

AU - Harris, L. E.

AU - Goodin, M. M.

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N2 - Coffee ringspot virus (CoRSV) a member of the proposed genus “Dichorhavirus”, was surveyed on commercial and research farms spanning an area responsible for the majority of Coffea arabica production in Brazil. Virus-infected plants were found at one hundred percent of locations (n = 45) sampled. All cultivars, regardless of cherry color, were found to serve as hosts, suggesting that there is limited resistance in commercially employed germplasm. Reverse transcription PCR analysis revealed that the virus is contained within symptomatic lesions, with little systemic spread throughout leaves. Phylogenetic analysis based on the ORF1 (nucleocapsid) gene identified a strong geo-spatial relationship among isolates, which clustered into three clades. Despite low genetic diversity among isolates, variation in symptom expression was observed in the experimental host Chenopodium quinoa. Our analyses support the hypothesis that the spread of CoRSV is constrained by the clonal expansion of thelytokous populations of Brevipalpus phoenicis. The widespread occurrence of this virus suggests that it is much more prevalent than previously thought.

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Detection and survey of coffee ringspot virus in Brazil

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