Abstract
Coffee ringspot virus (CoRSV) a member of the proposed genus “Dichorhavirus”, was surveyed on commercial and research farms spanning an area responsible for the majority of Coffea arabica production in Brazil. Virus-infected plants were found at one hundred percent of locations (n = 45) sampled. All cultivars, regardless of cherry color, were found to serve as hosts, suggesting that there is limited resistance in commercially employed germplasm. Reverse transcription PCR analysis revealed that the virus is contained within symptomatic lesions, with little systemic spread throughout leaves. Phylogenetic analysis based on the ORF1 (nucleocapsid) gene identified a strong geo-spatial relationship among isolates, which clustered into three clades. Despite low genetic diversity among isolates, variation in symptom expression was observed in the experimental host Chenopodium quinoa. Our analyses support the hypothesis that the spread of CoRSV is constrained by the clonal expansion of thelytokous populations of Brevipalpus phoenicis. The widespread occurrence of this virus suggests that it is much more prevalent than previously thought.
| Original language | English |
|---|---|
| Pages (from-to) | 335-343 |
| Number of pages | 9 |
| Journal | Archives of Virology |
| Volume | 161 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2016 |
Bibliographical note
Publisher Copyright:© 2015, Springer-Verlag Wien.
Funding
We thank the CNPq, CAPES, FAPEMIG, and NSF funding agencies for providing research grants to M.G. and A.F. We would also like to thank the farmers and cooperative workers who graciously provided access to their farms in support of this research. This publication recognizes CAPES Project No. A009, 1901133, and NSF-IOS-0749519).
| Funders | Funder number |
|---|---|
| Coordenação de Aperfeiçoamento de Pessoal de Nível Superior | |
| Conselho Nacional de Desenvolvimento Científico e Tecnológico | |
| Fundação de Amparo à Pesquisa do Estado de Minas Gerais | A009, NSF-IOS-0749519, 1901133 |
| U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China | 0749519 |
ASJC Scopus subject areas
- Virology