Detection of Borrelia burgdorferi gene expression during mammalian infection using transcriptional fusions that produce green fluorescent protein

Jennifer C. Miller; Kate von Lackum; Michael E. Woodman; Brian Stevenson

doi:10.1016/j.micpath.2006.04.004

Detection of Borrelia burgdorferi gene expression during mammalian infection using transcriptional fusions that produce green fluorescent protein

Jennifer C. Miller, Kate von Lackum, Michael E. Woodman, Brian Stevenson

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A novel, infectious Borrelia burgdorferi that expresses green fluorescent protein (GFP) was developed to examine the utility of this marker protein to label live bacteria during infection processes. Use of a borrelial erpAB promoter to direct gfp transcription supported previous indications that B. burgdorferi expresses erp genes during chronic mammalian infection and during acquisition by feeding ticks. Live bacteria fluoresced and were seen to be located extracellularly in infected mice and within midguts of infected ticks. These results indicate that transcriptional fusions between B. burgdorferi promoters and gfp can be useful tools to examine spirochete gene expression in vivo.

Original language	English
Pages (from-to)	43-47
Number of pages	5
Journal	Microbial Pathogenesis
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/j.micpath.2006.04.004
State	Published - Jul 2006

Bibliographical note

Funding Information:
These studies were funded by US National Institutes of Health Grants R01 AI44252 and T32 AI49795. We thank Kelly Babb, Sara Bair, Jerry Bowman, Tomasz Bykowski, Anne Cooley, Sarah Kearns, Natalie Mickelsen and Sean Riley for assistance.

Keywords

Borrelia burgdorferi
Gene fusion
Lyme disease
Spirochete

ASJC Scopus subject areas

Microbiology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.micpath.2006.04.004

Cite this

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title = "Detection of Borrelia burgdorferi gene expression during mammalian infection using transcriptional fusions that produce green fluorescent protein",

abstract = "A novel, infectious Borrelia burgdorferi that expresses green fluorescent protein (GFP) was developed to examine the utility of this marker protein to label live bacteria during infection processes. Use of a borrelial erpAB promoter to direct gfp transcription supported previous indications that B. burgdorferi expresses erp genes during chronic mammalian infection and during acquisition by feeding ticks. Live bacteria fluoresced and were seen to be located extracellularly in infected mice and within midguts of infected ticks. These results indicate that transcriptional fusions between B. burgdorferi promoters and gfp can be useful tools to examine spirochete gene expression in vivo.",

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author = "Miller, {Jennifer C.} and {von Lackum}, Kate and Woodman, {Michael E.} and Brian Stevenson",

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AU - Miller, Jennifer C.

AU - von Lackum, Kate

AU - Woodman, Michael E.

AU - Stevenson, Brian

N1 - Funding Information: These studies were funded by US National Institutes of Health Grants R01 AI44252 and T32 AI49795. We thank Kelly Babb, Sara Bair, Jerry Bowman, Tomasz Bykowski, Anne Cooley, Sarah Kearns, Natalie Mickelsen and Sean Riley for assistance.

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AB - A novel, infectious Borrelia burgdorferi that expresses green fluorescent protein (GFP) was developed to examine the utility of this marker protein to label live bacteria during infection processes. Use of a borrelial erpAB promoter to direct gfp transcription supported previous indications that B. burgdorferi expresses erp genes during chronic mammalian infection and during acquisition by feeding ticks. Live bacteria fluoresced and were seen to be located extracellularly in infected mice and within midguts of infected ticks. These results indicate that transcriptional fusions between B. burgdorferi promoters and gfp can be useful tools to examine spirochete gene expression in vivo.

KW - Borrelia burgdorferi

KW - Gene fusion

KW - Lyme disease

KW - Spirochete

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Detection of Borrelia burgdorferi gene expression during mammalian infection using transcriptional fusions that produce green fluorescent protein

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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