TY - CHAP
T1 - Detection of carbonylated proteins in 2-D SDS page separations
AU - Sultana, Rukhsana
AU - Newman, Shelley F.
AU - Huang, Quanzhen
AU - Allan Butterfield, D.
PY - 2008
Y1 - 2008
N2 - Protein carbonyls are an index of protein oxidation which, in turn, reflects the interplay of oxidative stress and degradation of oxidatively modified proteins. Protein carbonyls are increased in brain proteins in aging and age-related neurodegenerative disorders, including Alzheimer's disease. In this chapter, we outline methods to detect protein carbonyls following two dimensional-based separation of brain proteins.
AB - Protein carbonyls are an index of protein oxidation which, in turn, reflects the interplay of oxidative stress and degradation of oxidatively modified proteins. Protein carbonyls are increased in brain proteins in aging and age-related neurodegenerative disorders, including Alzheimer's disease. In this chapter, we outline methods to detect protein carbonyls following two dimensional-based separation of brain proteins.
KW - Imaging analysis
KW - SDS gels
KW - isoelectric focusing
KW - protein carbonyls
KW - two-dimensional western blots
UR - http://www.scopus.com/inward/record.url?scp=59249102807&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59249102807&partnerID=8YFLogxK
U2 - 10.1007/978-1-59745-129-1_11
DO - 10.1007/978-1-59745-129-1_11
M3 - Chapter
C2 - 19157015
AN - SCOPUS:59249102807
SN - 9781588298423
T3 - Methods in Molecular Biology
SP - 153
EP - 163
BT - Redox-Mediated Signal Transduction
A2 - Hancock, John
ER -