Detection of human cytomegalovirus in dental plaque from individual periodontal sites by real-time polymerase chain reaction

Darrin R. Combs, Elizabeth A. Reilly, Dolphus R. Dawson, Sergei A. Avdiushko, Robert J. Danaher, Craig S. Miller

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Objective: The aim was to evaluate three primer-probe sets and real-time polymerase chain reaction (PCR) for the detection of human cytomegalovirus (HCMV) in dental plaque from individual periodontal sites. Study design: Fifty subgingival plaque specimens from 13 healthy subjects (on average at least 2 healthy and 2 periodontal disease sites per subject) and 50 saliva specimens from 24 subjects, including 16 controls, were assessed using 3 primer-probe sets (polymerase [POL], glycoprotein B [gB], and US14) and real-time PCR. Kappa statistics were performed to measure agreement between the primer-probe sets. Results: There was excellent agreement between the gB and POL primers in the detection of HCMV (kappa statistic = 0.85 [95% confidence interval 0.71-0.99]), yielding a prevalence of 4% (2 out of 50) at individual periodontal disease sites and a similar rate of 8.8% (3 out of 34) in saliva. Conclusion: Human cytomegalovirus was infrequently detected in dental plaque. Of 3 primer-probe sets evaluated, those targeting the POL and gB genes were more accurate in the detection of HCMV than that targeting US14.

Original languageEnglish
Pages (from-to)840-844
Number of pages5
JournalOral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Volume106
Issue number6
DOIs
StatePublished - Dec 2008

Bibliographical note

Funding Information:
Supported by National Institutes of Health P20 RR020145 and M01RR02602 to the General Clinical Research Center.

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • General Dentistry

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