Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques

Mariano Carossino, Hon S. Ip, Juergen A. Richt, Kendra Shultz, Kimberly Harper, Alan T. Loynachan, Fabio Del Piero, Udeni B.R. Balasuriya

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

Original languageEnglish
Pages (from-to)2373-2377
Number of pages5
JournalArchives of Virology
Volume165
Issue number10
DOIs
StatePublished - Oct 1 2020

Bibliographical note

Publisher Copyright:
© 2020, Springer-Verlag GmbH Austria, part of Springer Nature.

ASJC Scopus subject areas

  • Virology

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