Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques

Mariano Carossino; Hon S. Ip; Juergen A. Richt; Kendra Shultz; Kimberly Harper; Alan T. Loynachan; Fabio Del Piero; Udeni B.R. Balasuriya

doi:10.1007/s00705-020-04737-w

Detection of SARS-CoV-2 by RNAscope^® in situ hybridization and immunohistochemistry techniques

Mariano Carossino, Hon S. Ip, Juergen A. Richt, Kendra Shultz, Kimberly Harper, Alan T. Loynachan, Fabio Del Piero, Udeni B.R. Balasuriya

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope^®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

Original language	English
Pages (from-to)	2373-2377
Number of pages	5
Journal	Archives of Virology
Volume	165
Issue number	10
DOIs	https://doi.org/10.1007/s00705-020-04737-w
State	Published - Oct 1 2020

Bibliographical note

Publisher Copyright:
© 2020, Springer-Verlag GmbH Austria, part of Springer Nature.

ASJC Scopus subject areas

Virology

Access to Document

10.1007/s00705-020-04737-w

Cite this

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abstract = "In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope{\textregistered}-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.",

author = "Mariano Carossino and Ip, {Hon S.} and Richt, {Juergen A.} and Kendra Shultz and Kimberly Harper and Loynachan, {Alan T.} and {Del Piero}, Fabio and Balasuriya, {Udeni B.R.}",

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AU - Carossino, Mariano

AU - Ip, Hon S.

AU - Richt, Juergen A.

AU - Shultz, Kendra

AU - Harper, Kimberly

AU - Loynachan, Alan T.

AU - Del Piero, Fabio

AU - Balasuriya, Udeni B.R.

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