TY - JOUR
T1 - Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques
AU - Carossino, Mariano
AU - Ip, Hon S.
AU - Richt, Juergen A.
AU - Shultz, Kendra
AU - Harper, Kimberly
AU - Loynachan, Alan T.
AU - Del Piero, Fabio
AU - Balasuriya, Udeni B.R.
N1 - Publisher Copyright:
© 2020, Springer-Verlag GmbH Austria, part of Springer Nature.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
AB - In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
UR - http://www.scopus.com/inward/record.url?scp=85089024400&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85089024400&partnerID=8YFLogxK
U2 - 10.1007/s00705-020-04737-w
DO - 10.1007/s00705-020-04737-w
M3 - Article
C2 - 32761270
AN - SCOPUS:85089024400
SN - 0304-8608
VL - 165
SP - 2373
EP - 2377
JO - Archives of Virology
JF - Archives of Virology
IS - 10
ER -