Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques

Mariano Carossino, Hon S. Ip, Juergen A. Richt, Kendra Shultz, Kimberly Harper, Alan T. Loynachan, Fabio Del Piero, Udeni B.R. Balasuriya

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

Original languageEnglish
Pages (from-to)2373-2377
Number of pages5
JournalArchives of Virology
Issue number10
StatePublished - Oct 1 2020

Bibliographical note

Funding Information:
This study was supported by Louisiana State University, School of Veterinary Medicine start-up fund (PG 002165) to Dr. Udeni B. R. Balasuriya, NIH-CEIRS, and NBAF Transition Funds from the State of Kansas and funds from the NIAID Centers of Excellence for Influenza Research and Surveillance under contract number HHSN 272201400006C to Dr. Juergen A. Richt. Acknowledgments

Funding Information:
The authors would like to acknowledge Ms. Cheryl Johnson and other members of the histopathology and immunohistochemistry section at the Louisiana Animal Disease Diagnostic Laboratory (LADDL), Louisiana State University (LSU) School of Veterinary Medicine (SVM), for assistance in slide preparation for RNAscope? and IHC assays, and Katy Griffin at the United States Department of the Interior, U.S. Geological Survey, National Wildlife Health Center for the SARS-CoV-2 virus culture. The use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

Publisher Copyright:
© 2020, Springer-Verlag GmbH Austria, part of Springer Nature.

ASJC Scopus subject areas

  • Virology


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