Determination of calmodulin by competitive binding assay

Harry LeVine, Naji Sahyoun, Pedro Cuatrecasas

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a tool to readily probe changes in calmodulin levels in cells and tissues as a function of pathophysiologic state.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalAnalytical Biochemistry
Issue number1
StatePublished - Jan 1986


  • I-calmodulin
  • cytoskeletal protein kinase
  • filtration

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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