Determination of calmodulin by competitive binding assay

Harry LeVine; Naji Sahyoun; Pedro Cuatrecasas

doi:10.1016/0003-2697(86)90139-9

Determination of calmodulin by competitive binding assay

Harry LeVine
, Naji Sahyoun
, Pedro Cuatrecasas

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Calmodulin levels in tissue or cellular extracts can be determined by competition with ¹²⁵I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a tool to readily probe changes in calmodulin levels in cells and tissues as a function of pathophysiologic state.

Original language	English
Pages (from-to)	183-188
Number of pages	6
Journal	Analytical Biochemistry
Volume	152
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(86)90139-9
State	Published - Jan 1986

Keywords

I-calmodulin
cytoskeletal protein kinase
filtration

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(86)90139-9

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title = "Determination of calmodulin by competitive binding assay",

abstract = "Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a tool to readily probe changes in calmodulin levels in cells and tissues as a function of pathophysiologic state.",

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author = "Harry LeVine and Naji Sahyoun and Pedro Cuatrecasas",

year = "1986",

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AU - LeVine, Harry

AU - Sahyoun, Naji

AU - Cuatrecasas, Pedro

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N2 - Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a tool to readily probe changes in calmodulin levels in cells and tissues as a function of pathophysiologic state.

AB - Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a tool to readily probe changes in calmodulin levels in cells and tissues as a function of pathophysiologic state.

KW - I-calmodulin

KW - cytoskeletal protein kinase

KW - filtration

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UR - https://www.scopus.com/pages/publications/0022617627#tab=citedBy

U2 - 10.1016/0003-2697(86)90139-9

DO - 10.1016/0003-2697(86)90139-9

M3 - Article

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SN - 0003-2697

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SP - 183

EP - 188

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Determination of calmodulin by competitive binding assay

Abstract

Keywords

ASJC Scopus subject areas

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