A sensitive and specific high-performance liquid chromatographic technique is described for the quantitative measurement of the synthetic glucocorticoid methylprednisolone in central nervous tissue (spinal cord) and plasma. Following intravenous administration, methylprednisolone is extracted from spinal cord tissue with diethyl ether-methylene chloride (60:40, v/v). The extract is washed sequentially with alkali, acid and water, concentrated, then chromatographed on an NH2 column using a mobile phase of methylene chloride-isopropanol (85:15, v/v). Steroid elution is monitored with an ultraviolet detector set at 254 nm. Such a system has a detection limit of 2.8 ng methylprednisolone. Extraction of methylprednisolone from spinal cord tissue is linear with tissue concentration and the recovery is around 70%. Endogenous hydrocortisone or other metabolites in the tissue do not interfere with the methylprednisolone peak. A description of the quantitation of methylprednisolone in cat lumbar spinal cord and plasma samples after single intravenous doses of methylprednisolone sodium succinate is given.
|Number of pages||7|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|State||Published - Jun 11 1982|
Bibliographical noteFunding Information:
The authors gratefWly acknowledge the technical assistance of Mrs. Brigitte Ii& and Ms. Cynthia Rowe and a gift of methylprednisolone sodium succinate ,Solu-Medrol) and methylprednisolone from Upjohn, Kalamazoo, MI, U.S.A. L’hisw ork was supported by USPHS grant MH 34111 to E.D.H.
ASJC Scopus subject areas
- Chemistry (all)