Determination of salmeterol in equine urine and serum

A. F. Lehner, C. G. Hughes, W. Karpiesiuk, F. C. Camargo, J. D. Harkins, W. E. Woods, J. Bosken, J. Boyles, A. Troppmann, T. Tobin

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Salmeterol is a β2-adrenergic agonist and an Association of Racing Commissioners International (ARCI) class 3 drug. Trade names of its xinafoate salt are Arial (Dompé), Salmetedur (Menarini), and Serevent (Glaxo). Salmeterol is routinely used to increase ease of breathing in race horses during their training. Due to its bronchodilating and central nervous system stimulant properties, its administration to a horse just prior to race time has the potential to affect the horse's performance, therefore a reliable method of analysis for this compound is necessary. This paper describes a method for the identification and quantitation of salmeterol in equine urine using liquid-liquid extraction followed by liquid chromatography and tandem mass spectrometry (LC-MS-MS). Urine salmeterol concentrations peaked at about 2 h post-dose following administration of 500 ug both intravenously and intratracheally at concentrations of 14 ng mL-1 and 4 ng ml -1, respectively. Serum concentrations ot 30 min were below the minimum level of quantitation.

Original languageEnglish
Pages (from-to)S79-S84
Issue numberSUPPL.
StatePublished - 2004


  • Bronchodilator
  • Column liquid chromatography
  • LC-MS-MS
  • Mass spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry


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