Abstract
trans-3-Hydroxycotinine is the major urinary metabolite of nicotine in man and can serve as an important biomarker of tobacco smoke exposure. A sensitive ELISA test for trans-3-hydroxycotinine was developed with an 1-50 for this nicotine biomarker of between 1.0-3.0 ng/ml. This ELISA test has about 10 fold less affinity for cotinine and 1000-fold less affinity for nicotine and other nicotine metabolites. No matrix effects were detectable in human saliva and relatively small matrix effects (I-50 for trans-3-hydroxycotinine, about 25 ng/ml) in urine was observed. The assay readily detected levels of apparent trans-3-hydroxycotinine in urine samples from smoke-exposed mice and rats. This ELISA is therefore a sensitive test for the determination of trans-3-hydroxycotinine in plasma, saliva, and urine samples from humans and animals, and can be used to monitor exposure to tobacco smoke or nicotine.
Original language | English |
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Pages (from-to) | 149-162 |
Number of pages | 14 |
Journal | ASTM Special Technical Publication |
Volume | 1306 |
State | Published - 1996 |
Keywords
- Cotinine
- Enzyme-Linked Immunosorbant Assay
- Metabolite
- Nicotine
- t-3-hydroxycotinine
ASJC Scopus subject areas
- Engineering (all)