Development and characterization of swine primary respiratory epithelial cells and their susceptibility to infection by four influenza virus types

Chithra C. Sreenivasan; Milton Thomas; Linto Antony; Tristen Wormstadt; Michael B. Hildreth; Dan Wang; Ben Hause; David H. Francis; Feng Li; Radhey S. Kaushik

doi:10.1016/j.virol.2018.12.016

Development and characterization of swine primary respiratory epithelial cells and their susceptibility to infection by four influenza virus types

Chithra C. Sreenivasan
, Milton Thomas
, Linto Antony
, Tristen Wormstadt
, Michael B. Hildreth
, Dan Wang
, Ben Hause
, David H. Francis
, Feng Li
, Radhey S. Kaushik

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Influenza viruses are a group of respiratory pathogens that have evolved into four different types: A, B, C, and D. A common feature is that all four types are capable of replicating and transmitting among pigs. Here, we describe the development of isogenous cell culture system from the swine respiratory tract to study influenza viruses. Phenotypic characterization of swine primary nasal turbinate, trachea and lung cells revealed high expression of cytokeratin and demonstrated tissue site dependent expression of tight junction proteins. Furthermore, lectin binding assay on these cells demonstrated higher levels of Sia2–6Gal than Sia2–3Gal receptors and supported the replication of influenza A, B, C, and D viruses to appreciable levels at both 33 and 37 °C, but replication competence was dependent on virus type or temperature used. Overall, these swine primary respiratory cells showed epithelial phenotype, which is suitable for studying the comparative biology and pathobiology of influenza viruses.

Original language	English
Pages (from-to)	152-163
Number of pages	12
Journal	Virology
Volume	528
DOIs	https://doi.org/10.1016/j.virol.2018.12.016
State	Published - Feb 2019

Bibliographical note

Publisher Copyright:
© 2018 Elsevier Inc.

Funding

Radhey S. Kaushik, Chithra C. Sreenivasan, Milton Thomas, Tristen Wormstadt and this research project were funded and supported by USDA NIFA SDSU Agricultural Experiment Station Hatch grant SD00H547-15 . Tristen Wormstadt is an undergraduate student. We acknowledge use of the SDSU-FGCF supported in part by NSF/EPSCoR Grant No. 0091948 and by the State of South Dakota. This study was also partially funded by NIH R15 grant number 1R15AI089690-01A1 . This study was also in part supported by SDSU-AES 4AH-477 , USDA/NIFA 2016-67016-24949 , NIH R21AI107379 , National Science Foundation/EPSCoR ( http://www.nsf.gov/od/iia/programs/epscor/index.jsp ) award IIA-1335423, and the State of South Dakota's Governor's Office of Economic Development as a South Dakota Research Innovation Center.

Funders	Funder number
SDSU AES	4AH-477, USDA/NIFA 2016-67016-24949
USDA NIFA SDSU Agricultural Experiment Station Hatch	SD00H547-15
National Science Foundation Arctic Social Science Program	IIA-1335423
National Institutes of Health (NIH)	1R15AI089690-01A1
National Institute of Allergy and Infectious Diseases	R21AI107379
Office of Experimental Program to Stimulate Competitive Research	0091948

Keywords

Influenza virus
Primary cells
Replication kinetics
Respiratory epithelial cells
Swine

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2018.12.016

Cite this

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title = "Development and characterization of swine primary respiratory epithelial cells and their susceptibility to infection by four influenza virus types",

abstract = "Influenza viruses are a group of respiratory pathogens that have evolved into four different types: A, B, C, and D. A common feature is that all four types are capable of replicating and transmitting among pigs. Here, we describe the development of isogenous cell culture system from the swine respiratory tract to study influenza viruses. Phenotypic characterization of swine primary nasal turbinate, trachea and lung cells revealed high expression of cytokeratin and demonstrated tissue site dependent expression of tight junction proteins. Furthermore, lectin binding assay on these cells demonstrated higher levels of Sia2{\textendash}6Gal than Sia2{\textendash}3Gal receptors and supported the replication of influenza A, B, C, and D viruses to appreciable levels at both 33 and 37 °C, but replication competence was dependent on virus type or temperature used. Overall, these swine primary respiratory cells showed epithelial phenotype, which is suitable for studying the comparative biology and pathobiology of influenza viruses.",

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doi = "10.1016/j.virol.2018.12.016",

language = "English",

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AU - Thomas, Milton

AU - Antony, Linto

AU - Wormstadt, Tristen

AU - Hildreth, Michael B.

AU - Wang, Dan

AU - Hause, Ben

AU - Francis, David H.

AU - Li, Feng

AU - Kaushik, Radhey S.

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KW - Replication kinetics

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Development and characterization of swine primary respiratory epithelial cells and their susceptibility to infection by four influenza virus types

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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