Development and evaluation of a sensitive, Diffusive Gradients in Thin-Films (DGT) method for determining microcystin-LR concentrations in freshwater and seawater

Elisa D'Angelo

doi:10.1016/j.hal.2019.101668

Development and evaluation of a sensitive, Diffusive Gradients in Thin-Films (DGT) method for determining microcystin-LR concentrations in freshwater and seawater

Elisa D'Angelo

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A Diffusive Gradients in Thin-Films (DGT) passive sampling technique was developed for microcystin-LR (MC-LR), one of the most common and toxic microcystins. Three types of resins (HP20, SP700, and XAD18) were evaluated for MC-LR uptake kinetics, capacities, and extraction efficiencies and simple procedures were developed for determining MC-LR concentration in binding disc extracts by Adda-ELISA (U.S. EPA Method 546). The XAD18-DGT/Adda-ELISA method had a 7-d deployment time detection limit of ≈0.05 μg/L and capacity of >250 μg/L of MC-LR in water samples which encompass U.S. EPA and WHO advisory concentrations for drinking and recreational waters. The XAD18-DGT/Adda-ELISA method determined time-averaged MC-LR concentrations in waters with wide ranging pH (4.9–8.3) and ionic strength (0.04–0.8 M) under well-stirred and quiescent conditions with 90–101% accuracy. In addition to high sensitivity and accuracy, the method is simple, inexpensive, and applicable for determining MC-LR and related MCs concentrations in waterbodies with wide ranging chemical characteristics and hydrodynamic conditions.

Original language	English
Article number	101668
Journal	Harmful Algae
Volume	89
DOIs	https://doi.org/10.1016/j.hal.2019.101668
State	Published - Nov 2019

Bibliographical note

Publisher Copyright:
© 2019 Elsevier B.V.

Funding

The author expresses her sincere appreciation for research support from the Hatch Multistate Research Fund (MRF) provided by National Institute of Food and Agriculture for project W3170-Beneficial Reuse of Residuals and Reclaimed Water: Impact on Soil Ecosystem and Human Health. [CG]

Funders
Melanoma Research Alliance Foundation
US Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative

Keywords

Adda-enzyme-linked immuno-sorbent assay (ELISA)
Algal toxins
Diffusive boundary layer (DBL)
Diffusive gradients in thin-films (DGT)
Passive sampling
U.S. EPA method 546

ASJC Scopus subject areas

Aquatic Science
Plant Science

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10.1016/j.hal.2019.101668

Cite this

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title = "Development and evaluation of a sensitive, Diffusive Gradients in Thin-Films (DGT) method for determining microcystin-LR concentrations in freshwater and seawater",

abstract = "A Diffusive Gradients in Thin-Films (DGT) passive sampling technique was developed for microcystin-LR (MC-LR), one of the most common and toxic microcystins. Three types of resins (HP20, SP700, and XAD18) were evaluated for MC-LR uptake kinetics, capacities, and extraction efficiencies and simple procedures were developed for determining MC-LR concentration in binding disc extracts by Adda-ELISA (U.S. EPA Method 546). The XAD18-DGT/Adda-ELISA method had a 7-d deployment time detection limit of ≈0.05 μg/L and capacity of >250 μg/L of MC-LR in water samples which encompass U.S. EPA and WHO advisory concentrations for drinking and recreational waters. The XAD18-DGT/Adda-ELISA method determined time-averaged MC-LR concentrations in waters with wide ranging pH (4.9–8.3) and ionic strength (0.04–0.8 M) under well-stirred and quiescent conditions with 90–101\% accuracy. In addition to high sensitivity and accuracy, the method is simple, inexpensive, and applicable for determining MC-LR and related MCs concentrations in waterbodies with wide ranging chemical characteristics and hydrodynamic conditions.",

keywords = "Adda-enzyme-linked immuno-sorbent assay (ELISA), Algal toxins, Diffusive boundary layer (DBL), Diffusive gradients in thin-films (DGT), Passive sampling, U.S. EPA method 546",

author = "Elisa D'Angelo",

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year = "2019",

month = nov,

doi = "10.1016/j.hal.2019.101668",

language = "English",

volume = "89",

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N2 - A Diffusive Gradients in Thin-Films (DGT) passive sampling technique was developed for microcystin-LR (MC-LR), one of the most common and toxic microcystins. Three types of resins (HP20, SP700, and XAD18) were evaluated for MC-LR uptake kinetics, capacities, and extraction efficiencies and simple procedures were developed for determining MC-LR concentration in binding disc extracts by Adda-ELISA (U.S. EPA Method 546). The XAD18-DGT/Adda-ELISA method had a 7-d deployment time detection limit of ≈0.05 μg/L and capacity of >250 μg/L of MC-LR in water samples which encompass U.S. EPA and WHO advisory concentrations for drinking and recreational waters. The XAD18-DGT/Adda-ELISA method determined time-averaged MC-LR concentrations in waters with wide ranging pH (4.9–8.3) and ionic strength (0.04–0.8 M) under well-stirred and quiescent conditions with 90–101% accuracy. In addition to high sensitivity and accuracy, the method is simple, inexpensive, and applicable for determining MC-LR and related MCs concentrations in waterbodies with wide ranging chemical characteristics and hydrodynamic conditions.

AB - A Diffusive Gradients in Thin-Films (DGT) passive sampling technique was developed for microcystin-LR (MC-LR), one of the most common and toxic microcystins. Three types of resins (HP20, SP700, and XAD18) were evaluated for MC-LR uptake kinetics, capacities, and extraction efficiencies and simple procedures were developed for determining MC-LR concentration in binding disc extracts by Adda-ELISA (U.S. EPA Method 546). The XAD18-DGT/Adda-ELISA method had a 7-d deployment time detection limit of ≈0.05 μg/L and capacity of >250 μg/L of MC-LR in water samples which encompass U.S. EPA and WHO advisory concentrations for drinking and recreational waters. The XAD18-DGT/Adda-ELISA method determined time-averaged MC-LR concentrations in waters with wide ranging pH (4.9–8.3) and ionic strength (0.04–0.8 M) under well-stirred and quiescent conditions with 90–101% accuracy. In addition to high sensitivity and accuracy, the method is simple, inexpensive, and applicable for determining MC-LR and related MCs concentrations in waterbodies with wide ranging chemical characteristics and hydrodynamic conditions.

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KW - Algal toxins

KW - Diffusive boundary layer (DBL)

KW - Diffusive gradients in thin-films (DGT)

KW - Passive sampling

KW - U.S. EPA method 546

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DO - 10.1016/j.hal.2019.101668

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SN - 1568-9883

VL - 89

JO - Harmful Algae

JF - Harmful Algae

M1 - 101668

ER -

Development and evaluation of a sensitive, Diffusive Gradients in Thin-Films (DGT) method for determining microcystin-LR concentrations in freshwater and seawater

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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