Development of a LC/MS/MS method to analyze butyrylcholinesterase inhibition resulting from multiple pesticide exposure

Jinchun Sun; Bert C. Lynn

doi:10.1016/j.jchromb.2009.09.009

Development of a LC/MS/MS method to analyze butyrylcholinesterase inhibition resulting from multiple pesticide exposure

Jinchun Sun, Bert C. Lynn

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A hybrid LC/MS/MS and proteomics method was developed for the assessment of multiple pesticide exposure. The methodology was based on the analysis of tryptic peptides resulting from inhibited butyrylcholinesterase (BChE) after exposure to pesticides including organophosphates (OPs) and carbamates (CBs). The primary advantage of the assay was its ability to simultaneously examine multiple pesticide exposures in a single analytical experiment. Application of tandem and MS³ techniques provided identities of the inhibiting pesticide, confirmation and localization of the site of inhibition and relative quantification of phosphorylated peptides present in tryptic digests of equine BChE (eBChE).

Original language	English
Pages (from-to)	3681-3685
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	29
DOIs	https://doi.org/10.1016/j.jchromb.2009.09.009
State	Published - Nov 1 2009

Keywords

Butyrylcholinesterase
Carbamate
LC/MS/MS
Organophosphate
Proteomics

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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10.1016/j.jchromb.2009.09.009

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title = "Development of a LC/MS/MS method to analyze butyrylcholinesterase inhibition resulting from multiple pesticide exposure",

abstract = "A hybrid LC/MS/MS and proteomics method was developed for the assessment of multiple pesticide exposure. The methodology was based on the analysis of tryptic peptides resulting from inhibited butyrylcholinesterase (BChE) after exposure to pesticides including organophosphates (OPs) and carbamates (CBs). The primary advantage of the assay was its ability to simultaneously examine multiple pesticide exposures in a single analytical experiment. Application of tandem and MS3 techniques provided identities of the inhibiting pesticide, confirmation and localization of the site of inhibition and relative quantification of phosphorylated peptides present in tryptic digests of equine BChE (eBChE).",

keywords = "Butyrylcholinesterase, Carbamate, LC/MS/MS, Organophosphate, Proteomics",

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AU - Sun, Jinchun

AU - Lynn, Bert C.

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N2 - A hybrid LC/MS/MS and proteomics method was developed for the assessment of multiple pesticide exposure. The methodology was based on the analysis of tryptic peptides resulting from inhibited butyrylcholinesterase (BChE) after exposure to pesticides including organophosphates (OPs) and carbamates (CBs). The primary advantage of the assay was its ability to simultaneously examine multiple pesticide exposures in a single analytical experiment. Application of tandem and MS3 techniques provided identities of the inhibiting pesticide, confirmation and localization of the site of inhibition and relative quantification of phosphorylated peptides present in tryptic digests of equine BChE (eBChE).

AB - A hybrid LC/MS/MS and proteomics method was developed for the assessment of multiple pesticide exposure. The methodology was based on the analysis of tryptic peptides resulting from inhibited butyrylcholinesterase (BChE) after exposure to pesticides including organophosphates (OPs) and carbamates (CBs). The primary advantage of the assay was its ability to simultaneously examine multiple pesticide exposures in a single analytical experiment. Application of tandem and MS3 techniques provided identities of the inhibiting pesticide, confirmation and localization of the site of inhibition and relative quantification of phosphorylated peptides present in tryptic digests of equine BChE (eBChE).

KW - Butyrylcholinesterase

KW - Carbamate

KW - LC/MS/MS

KW - Organophosphate

KW - Proteomics

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

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ER -

Development of a LC/MS/MS method to analyze butyrylcholinesterase inhibition resulting from multiple pesticide exposure

Abstract

Keywords

ASJC Scopus subject areas

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