The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization.
|Number of pages||4|
|State||Published - Nov 1 2011|
Bibliographical noteFunding Information:
This work was supported by funding from the NIH ( AI52218 ) and the Laura and Edward Kremers Chair in Natural Products Chemistry (J.S.T.).
- Sugar nucleotide
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology