Development of a universal glycosyltransferase assay amenable to high-throughput formats

Hyun Soo Lee, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalAnalytical Biochemistry
Issue number1
StatePublished - Nov 1 2011

Bibliographical note

Funding Information:
This work was supported by funding from the NIH ( AI52218 ) and the Laura and Edward Kremers Chair in Natural Products Chemistry (J.S.T.).


  • Carbohydrate
  • Engineering
  • Enzyme
  • Evolution
  • Glycosyltransferase
  • Sugar nucleotide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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