Development of near-isogenic lines in a parthenogenetically reproduced thrips species, Frankliniella occidentalis

Guangdi Yuan, Yanran Wan, Xiaoyu Li, Bingqing He, Youjun Zhang, Baoyun Xu, Shaoli Wang, Wen Xie, Xuguo Zhou, Qingjun Wu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Although near-isogenic lines (NILs) can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. Here, through multiple rounds of backcrossing and spinosad screening, we generated spinosad resistant NILs in the western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), with a haplo-diploid reproduction system. The resultant F. occidentalis NIL-R strain maintained a resistance ratio over 30,000-fold, which was comparable to its parental resistant strain, Spin-R. More importantly, F. occidentalis NIL-R shared 98.90% genetic similarity with its susceptible parental strain Ivf03. By developing this toolset, we are able to segregate individual resistance and facilitate the mechanistic study of insecticide resistances in phloem-feeding arthropods, a group of devastating pest species reproducing sexually as well as asexually.

Original languageEnglish
Article number130
JournalFrontiers in Physiology
Volume8
Issue numberMAR
DOIs
StatePublished - Mar 13 2017

Bibliographical note

Publisher Copyright:
© 2017 Yuan, Wan, Li, He, Zhang, Xu, Wang, Xie, Zhou and Wu.

Keywords

  • Frankliniella occidentalis
  • ISSR
  • Insecticide resistance
  • Near-isogenic lines
  • Spinosad

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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