Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin

S. Chattopadhyay; A. K. Srivastava; S. S. Bhojwani; V. S. Bisaria

doi:10.1023/A:1013704116860

Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin

S. Chattopadhyay, A. K. Srivastava, S. S. Bhojwani, V. S. Bisaria

Microbiology, Immunology, and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

A suspension culture of Podophyllum hexandrum was established. As the cultures grew, reduction in cell viability, biomass and product yield were associated with browning of culture medium, clumping of cells and drop in medium pH. Supplementation of the medium with both polyvinylpyrrollidone (PVP) and pectinase eliminated these problems. PVP at 10 g 1^-1 was optimum for both growth of and product formation in P. hexandrum suspension cultures.

Original language	English
Pages (from-to)	2063-2066
Number of pages	4
Journal	Biotechnology Letters
Volume	23
Issue number	24
DOIs	https://doi.org/10.1023/A:1013704116860
State	Published - 2001

Keywords

Podophyllotoxin
Podophyllum hexandrum
Suspension culture

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1023/A:1013704116860

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title = "Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin",

abstract = "A suspension culture of Podophyllum hexandrum was established. As the cultures grew, reduction in cell viability, biomass and product yield were associated with browning of culture medium, clumping of cells and drop in medium pH. Supplementation of the medium with both polyvinylpyrrollidone (PVP) and pectinase eliminated these problems. PVP at 10 g 1-1 was optimum for both growth of and product formation in P. hexandrum suspension cultures.",

keywords = "Podophyllotoxin, Podophyllum hexandrum, Suspension culture",

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AU - Chattopadhyay, S.

AU - Srivastava, A. K.

AU - Bhojwani, S. S.

AU - Bisaria, V. S.

PY - 2001

Y1 - 2001

N2 - A suspension culture of Podophyllum hexandrum was established. As the cultures grew, reduction in cell viability, biomass and product yield were associated with browning of culture medium, clumping of cells and drop in medium pH. Supplementation of the medium with both polyvinylpyrrollidone (PVP) and pectinase eliminated these problems. PVP at 10 g 1-1 was optimum for both growth of and product formation in P. hexandrum suspension cultures.

AB - A suspension culture of Podophyllum hexandrum was established. As the cultures grew, reduction in cell viability, biomass and product yield were associated with browning of culture medium, clumping of cells and drop in medium pH. Supplementation of the medium with both polyvinylpyrrollidone (PVP) and pectinase eliminated these problems. PVP at 10 g 1-1 was optimum for both growth of and product formation in P. hexandrum suspension cultures.

KW - Podophyllotoxin

KW - Podophyllum hexandrum

KW - Suspension culture

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin

Abstract

Keywords

ASJC Scopus subject areas

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