Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin

S. Chattopadhyay, A. K. Srivastava, S. S. Bhojwani, V. S. Bisaria

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

A suspension culture of Podophyllum hexandrum was established. As the cultures grew, reduction in cell viability, biomass and product yield were associated with browning of culture medium, clumping of cells and drop in medium pH. Supplementation of the medium with both polyvinylpyrrollidone (PVP) and pectinase eliminated these problems. PVP at 10 g 1-1 was optimum for both growth of and product formation in P. hexandrum suspension cultures.

Original languageEnglish
Pages (from-to)2063-2066
Number of pages4
JournalBiotechnology Letters
Volume23
Issue number24
DOIs
StatePublished - 2001

Keywords

  • Podophyllotoxin
  • Podophyllum hexandrum
  • Suspension culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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