Developmental and hormonal regulation of juvenile hormone esterase gene in Drosophila melanogaster

Damu R. Kethidi; Zhiyong Xi; Subba R. Palli

doi:10.1016/j.jinsphys.2004.12.007

Developmental and hormonal regulation of juvenile hormone esterase gene in Drosophila melanogaster

Damu R. Kethidi, Zhiyong Xi, Subba R. Palli

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to study developmental expression and hormonal regulation of the juvenile hormone esterase gene (DmJhe) in the fruit fly, Drosophila melanogaster. The levels of DmJhe mRNA were low during the embryonic stage. A peak of Dmjhe mRNA was detected in the first, second and third instar larvae. The Dmjhe mRNA levels also increased soon after pupal ecdysis. The Dmjhe mRNA was detected in both male and female adult flies. The peaks of Dmjhe mRNA observed in the larvae coincided with the peaks of juvenile hormone (JH). In contrast, the mRNA for ecdysone-induced transcription factor, Drosophila hormone receptor 3 (DHR3) showed peaks of expression that coincided with the ecdysteroid peaks in embryo, larva and pupa. JH III induced Dmjhe mRNA but not DHR3 mRNA in explanted tissues cultured in Grace's medium. 20-hydroxyecdysone induced DHR3 mRNA and suppressed JH III induction of DmJhe mRNA. These studies show that the expression of jhe in D. melanogaster is regulated by both JH and 20E.

Original language	English
Pages (from-to)	393-400
Number of pages	8
Journal	Journal of Insect Physiology
Volume	51
Issue number	4 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.jinsphys.2004.12.007
State	Published - Apr 2005

Bibliographical note

Funding Information:
Supported by a grant from National Science Foundation (IBN-0421856). We than Mr. Jason Pieratt of University of Kentucky for help with statistical analysis. This is paper number 04-08-125 of the University of Kentucky Agricultural Experimental Station.

Funding

Supported by a grant from National Science Foundation (IBN-0421856). We than Mr. Jason Pieratt of University of Kentucky for help with statistical analysis. This is paper number 04-08-125 of the University of Kentucky Agricultural Experimental Station.

Funders	Funder number
National Science Foundation (NSF)	IBN-0421856

Keywords

Ecdysone
Gene regulation and hormone action
Juvenile hormone

ASJC Scopus subject areas

Physiology
Insect Science

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10.1016/j.jinsphys.2004.12.007

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title = "Developmental and hormonal regulation of juvenile hormone esterase gene in Drosophila melanogaster",

abstract = "Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to study developmental expression and hormonal regulation of the juvenile hormone esterase gene (DmJhe) in the fruit fly, Drosophila melanogaster. The levels of DmJhe mRNA were low during the embryonic stage. A peak of Dmjhe mRNA was detected in the first, second and third instar larvae. The Dmjhe mRNA levels also increased soon after pupal ecdysis. The Dmjhe mRNA was detected in both male and female adult flies. The peaks of Dmjhe mRNA observed in the larvae coincided with the peaks of juvenile hormone (JH). In contrast, the mRNA for ecdysone-induced transcription factor, Drosophila hormone receptor 3 (DHR3) showed peaks of expression that coincided with the ecdysteroid peaks in embryo, larva and pupa. JH III induced Dmjhe mRNA but not DHR3 mRNA in explanted tissues cultured in Grace's medium. 20-hydroxyecdysone induced DHR3 mRNA and suppressed JH III induction of DmJhe mRNA. These studies show that the expression of jhe in D. melanogaster is regulated by both JH and 20E.",

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author = "Kethidi, {Damu R.} and Zhiyong Xi and Palli, {Subba R.}",

note = "Funding Information: Supported by a grant from National Science Foundation (IBN-0421856). We than Mr. Jason Pieratt of University of Kentucky for help with statistical analysis. This is paper number 04-08-125 of the University of Kentucky Agricultural Experimental Station. ",

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AU - Kethidi, Damu R.

AU - Xi, Zhiyong

AU - Palli, Subba R.

N1 - Funding Information: Supported by a grant from National Science Foundation (IBN-0421856). We than Mr. Jason Pieratt of University of Kentucky for help with statistical analysis. This is paper number 04-08-125 of the University of Kentucky Agricultural Experimental Station.

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N2 - Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to study developmental expression and hormonal regulation of the juvenile hormone esterase gene (DmJhe) in the fruit fly, Drosophila melanogaster. The levels of DmJhe mRNA were low during the embryonic stage. A peak of Dmjhe mRNA was detected in the first, second and third instar larvae. The Dmjhe mRNA levels also increased soon after pupal ecdysis. The Dmjhe mRNA was detected in both male and female adult flies. The peaks of Dmjhe mRNA observed in the larvae coincided with the peaks of juvenile hormone (JH). In contrast, the mRNA for ecdysone-induced transcription factor, Drosophila hormone receptor 3 (DHR3) showed peaks of expression that coincided with the ecdysteroid peaks in embryo, larva and pupa. JH III induced Dmjhe mRNA but not DHR3 mRNA in explanted tissues cultured in Grace's medium. 20-hydroxyecdysone induced DHR3 mRNA and suppressed JH III induction of DmJhe mRNA. These studies show that the expression of jhe in D. melanogaster is regulated by both JH and 20E.

AB - Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to study developmental expression and hormonal regulation of the juvenile hormone esterase gene (DmJhe) in the fruit fly, Drosophila melanogaster. The levels of DmJhe mRNA were low during the embryonic stage. A peak of Dmjhe mRNA was detected in the first, second and third instar larvae. The Dmjhe mRNA levels also increased soon after pupal ecdysis. The Dmjhe mRNA was detected in both male and female adult flies. The peaks of Dmjhe mRNA observed in the larvae coincided with the peaks of juvenile hormone (JH). In contrast, the mRNA for ecdysone-induced transcription factor, Drosophila hormone receptor 3 (DHR3) showed peaks of expression that coincided with the ecdysteroid peaks in embryo, larva and pupa. JH III induced Dmjhe mRNA but not DHR3 mRNA in explanted tissues cultured in Grace's medium. 20-hydroxyecdysone induced DHR3 mRNA and suppressed JH III induction of DmJhe mRNA. These studies show that the expression of jhe in D. melanogaster is regulated by both JH and 20E.

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Developmental and hormonal regulation of juvenile hormone esterase gene in Drosophila melanogaster

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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