Developmental changes in rat cardiac titin/connectin: Transitions in normal animals and in mutants with a delayed pattern of isoform transition

Marion L. Greaser, Paul R. Krzesinski, Chad M. Warren, Brian Kirkpatrick, Kenneth S. Campbell, Richard L. Moss

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Rat cardiac titin undergoes developmental changes in isoform expression during the period from late embryonic through the first 20-25 days of life. At least five size classes of titin isoforms have been identified using SDS agarose gel electrophoresis. The longest normal isoform is expressed in the embryonic stages, and it is progressively replaced with increasingly smaller versions. The isoform switching is consistent with changes in resting tension from lower values in one-day neonates to higher levels in adult myocytes. Considerable micro-heterogeneity in alternative splicing patterns also was found, particularly in the N2BA PEVK region of human, rat, and dog ventricle. A rat mutation has been identified in which the embryonic-neonatal titin isoform transitions are markedly delayed. These mutant animals may prove useful for examining the role of titin in stretch-activated signal transduction and in the Frank-Starling relationship.

Original languageEnglish
Pages (from-to)325-332
Number of pages8
JournalJournal of Muscle Research and Cell Motility
Volume26
Issue number6-8
DOIs
StatePublished - Dec 2005

Bibliographical note

Funding Information:
This work was supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison and by grants from the National Institutes of Health (HL47053; HL62466; HL77196).

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cell Biology

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