Developmental expression and stress induction of glutathione S-transferase in the spruce budworm, Choristoneura fumiferana

Qi Li Feng; Kenneth G. Davey; Anthony S.D. Pang; Tim R. Ladd; Arthur Retnakaran; Bill L. Tomkins; Sichun Zheng; Subba R. Palli

doi:10.1016/S0022-1910(00)00093-7

Developmental expression and stress induction of glutathione S-transferase in the spruce budworm, Choristoneura fumiferana

Qi Li Feng
, Kenneth G. Davey
, Anthony S.D. Pang
, Tim R. Ladd
, Arthur Retnakaran
, Bill L. Tomkins
, Sichun Zheng
, Subba R. Palli

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Developmental and stress-induced expression of Choristoneura fumiferana glutathione S-transferase (CfGST) mRNA and protein were examined using Northern blots and Western blots. High levels of CfGST mRNA and protein were detected in 1st instar larvae and diapausing 2nd instar larvae. Expression of CfGST gradually decreased during larval development from 3rd to 5th instar, after which the expression increased once again, reaching peak levels in 6th instar larvae. CfGST mRNA and protein were undetectable in the pupal stage. Exposure to low temperature did not induce an increase in CfGST expression. Feeding on balsam fir foliage resulted in an increase in the expression of CfGST as compared to larvae that fed on artificial diet. The bacterial insecticide, Bacillus thuringiensis delta-endotoxin (Bt), the non-steroidal ecdysone analog, tebufenozide, and the synthetic pyrethroid, permethrin, induced the expression of CfGST mRNA in 5th instar larvae, whereas the chitin synthesis inhibitor, diflubenzuron, did not have any such effect. These results suggest that CfGST plays an important role in detoxifying various allelochemicals and insecticides in the spruce budworm. The developmental expression pattern strongly suggests that in addition to detoxification, CfGST might be involved in other functions. Crown Copyright (C) 2000 Published by Elsevier Science Ltd.

Original language	English
Pages (from-to)	1-10
Number of pages	10
Journal	Journal of Insect Physiology
Volume	47
Issue number	1
DOIs	https://doi.org/10.1016/S0022-1910(00)00093-7
State	Published - 2001

Bibliographical note

Funding Information:
This research was supported in part by the National Biotechnology Strategy Fund and the Science and Technology Opportunities Fund of Canadian Forest Service to the Biotechnology group at the Great Lakes Forestry Centre and grants from the Natural Sciences and Engineering Research Council of Canada to K.G. Davey of York University.

Funding

This research was supported in part by the National Biotechnology Strategy Fund and the Science and Technology Opportunities Fund of Canadian Forest Service to the Biotechnology group at the Great Lakes Forestry Centre and grants from the Natural Sciences and Engineering Research Council of Canada to K.G. Davey of York University.

Funders
National Biotechnology Strategy Fund
Natural Sciences and Engineering Research Council of Canada
York University, New York, New York, USA bbbUniversity of Rochester, Rochester, New York
Canadian Forest Service and Science and Technology Opportunities Fund

Keywords

Allelochemical
Bacillus thuringiensis
Detoxification
Diflubenzuron
Insecticide
Permethrin
Tebufenozide

ASJC Scopus subject areas

Physiology
Insect Science

Access to Document

10.1016/S0022-1910(00)00093-7

Cite this

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title = "Developmental expression and stress induction of glutathione S-transferase in the spruce budworm, Choristoneura fumiferana",

abstract = "Developmental and stress-induced expression of Choristoneura fumiferana glutathione S-transferase (CfGST) mRNA and protein were examined using Northern blots and Western blots. High levels of CfGST mRNA and protein were detected in 1st instar larvae and diapausing 2nd instar larvae. Expression of CfGST gradually decreased during larval development from 3rd to 5th instar, after which the expression increased once again, reaching peak levels in 6th instar larvae. CfGST mRNA and protein were undetectable in the pupal stage. Exposure to low temperature did not induce an increase in CfGST expression. Feeding on balsam fir foliage resulted in an increase in the expression of CfGST as compared to larvae that fed on artificial diet. The bacterial insecticide, Bacillus thuringiensis delta-endotoxin (Bt), the non-steroidal ecdysone analog, tebufenozide, and the synthetic pyrethroid, permethrin, induced the expression of CfGST mRNA in 5th instar larvae, whereas the chitin synthesis inhibitor, diflubenzuron, did not have any such effect. These results suggest that CfGST plays an important role in detoxifying various allelochemicals and insecticides in the spruce budworm. The developmental expression pattern strongly suggests that in addition to detoxification, CfGST might be involved in other functions. Crown Copyright (C) 2000 Published by Elsevier Science Ltd.",

keywords = "Allelochemical, Bacillus thuringiensis, Detoxification, Diflubenzuron, Insecticide, Permethrin, Tebufenozide",

author = "Feng, \{Qi Li\} and Davey, \{Kenneth G.\} and \{S.D. Pang\}, Anthony and Ladd, \{Tim R.\} and Arthur Retnakaran and Tomkins, \{Bill L.\} and Sichun Zheng and Palli, \{Subba R.\}",

note = "Funding Information: This research was supported in part by the National Biotechnology Strategy Fund and the Science and Technology Opportunities Fund of Canadian Forest Service to the Biotechnology group at the Great Lakes Forestry Centre and grants from the Natural Sciences and Engineering Research Council of Canada to K.G. Davey of York University.",

year = "2001",

doi = "10.1016/S0022-1910(00)00093-7",

language = "English",

volume = "47",

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AU - Feng, Qi Li

AU - Davey, Kenneth G.

AU - S.D. Pang, Anthony

AU - Ladd, Tim R.

AU - Retnakaran, Arthur

AU - Tomkins, Bill L.

AU - Zheng, Sichun

AU - Palli, Subba R.

N1 - Funding Information: This research was supported in part by the National Biotechnology Strategy Fund and the Science and Technology Opportunities Fund of Canadian Forest Service to the Biotechnology group at the Great Lakes Forestry Centre and grants from the Natural Sciences and Engineering Research Council of Canada to K.G. Davey of York University.

PY - 2001

Y1 - 2001

N2 - Developmental and stress-induced expression of Choristoneura fumiferana glutathione S-transferase (CfGST) mRNA and protein were examined using Northern blots and Western blots. High levels of CfGST mRNA and protein were detected in 1st instar larvae and diapausing 2nd instar larvae. Expression of CfGST gradually decreased during larval development from 3rd to 5th instar, after which the expression increased once again, reaching peak levels in 6th instar larvae. CfGST mRNA and protein were undetectable in the pupal stage. Exposure to low temperature did not induce an increase in CfGST expression. Feeding on balsam fir foliage resulted in an increase in the expression of CfGST as compared to larvae that fed on artificial diet. The bacterial insecticide, Bacillus thuringiensis delta-endotoxin (Bt), the non-steroidal ecdysone analog, tebufenozide, and the synthetic pyrethroid, permethrin, induced the expression of CfGST mRNA in 5th instar larvae, whereas the chitin synthesis inhibitor, diflubenzuron, did not have any such effect. These results suggest that CfGST plays an important role in detoxifying various allelochemicals and insecticides in the spruce budworm. The developmental expression pattern strongly suggests that in addition to detoxification, CfGST might be involved in other functions. Crown Copyright (C) 2000 Published by Elsevier Science Ltd.

AB - Developmental and stress-induced expression of Choristoneura fumiferana glutathione S-transferase (CfGST) mRNA and protein were examined using Northern blots and Western blots. High levels of CfGST mRNA and protein were detected in 1st instar larvae and diapausing 2nd instar larvae. Expression of CfGST gradually decreased during larval development from 3rd to 5th instar, after which the expression increased once again, reaching peak levels in 6th instar larvae. CfGST mRNA and protein were undetectable in the pupal stage. Exposure to low temperature did not induce an increase in CfGST expression. Feeding on balsam fir foliage resulted in an increase in the expression of CfGST as compared to larvae that fed on artificial diet. The bacterial insecticide, Bacillus thuringiensis delta-endotoxin (Bt), the non-steroidal ecdysone analog, tebufenozide, and the synthetic pyrethroid, permethrin, induced the expression of CfGST mRNA in 5th instar larvae, whereas the chitin synthesis inhibitor, diflubenzuron, did not have any such effect. These results suggest that CfGST plays an important role in detoxifying various allelochemicals and insecticides in the spruce budworm. The developmental expression pattern strongly suggests that in addition to detoxification, CfGST might be involved in other functions. Crown Copyright (C) 2000 Published by Elsevier Science Ltd.

KW - Allelochemical

KW - Bacillus thuringiensis

KW - Detoxification

KW - Diflubenzuron

KW - Insecticide

KW - Permethrin

KW - Tebufenozide

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DO - 10.1016/S0022-1910(00)00093-7

M3 - Article

AN - SCOPUS:0035225695

SN - 0022-1910

VL - 47

SP - 1

EP - 10

JO - Journal of Insect Physiology

JF - Journal of Insect Physiology

IS - 1

ER -

Developmental expression and stress induction of glutathione S-transferase in the spruce budworm, Choristoneura fumiferana

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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