TY - JOUR
T1 - Diabetic modulation of the temperature kinetics properties of cytochrome oxidase activity in rat brain mitochondria
AU - Katyare, Surendra S.
AU - Patel, Samir P.
AU - Modi, Hiren R.
PY - 2008/3
Y1 - 2008/3
N2 - The effects of alloxan-diabetes and subsequent treatment with insulin on temperature kinetics properties of cytochrome oxidase activity from rat brain mitochondria were examined. The enzyme activity decreased only at the late stage of diabetes which was not normalized by insulin treatment; however at early stage of diabetes hyper-stimulation occurred. In the control animals the Arrhenius plot was chair shaped with three energies of (E1, E 2 and E3) and two phase transition temperatures (T t1 and Tt2). At early diabetic stage the Arrhenius plot became biphasic and E1 and E2 decreased; insulin treatment reversed chair-shaped pattern with increase in E2. These changes correlated with transient changes in the phospholipids profiles especially decreased acidic phospholipids. The temperature kinetics parameters were minimally affected at the late stage of diabetes or by insulin treatment. Thus at the late stage the brain tissue seems to have readjusted to its insulin homeostasis.
AB - The effects of alloxan-diabetes and subsequent treatment with insulin on temperature kinetics properties of cytochrome oxidase activity from rat brain mitochondria were examined. The enzyme activity decreased only at the late stage of diabetes which was not normalized by insulin treatment; however at early stage of diabetes hyper-stimulation occurred. In the control animals the Arrhenius plot was chair shaped with three energies of (E1, E 2 and E3) and two phase transition temperatures (T t1 and Tt2). At early diabetic stage the Arrhenius plot became biphasic and E1 and E2 decreased; insulin treatment reversed chair-shaped pattern with increase in E2. These changes correlated with transient changes in the phospholipids profiles especially decreased acidic phospholipids. The temperature kinetics parameters were minimally affected at the late stage of diabetes or by insulin treatment. Thus at the late stage the brain tissue seems to have readjusted to its insulin homeostasis.
KW - Alloxan-diabetes
KW - Cytochrome oxidase
KW - Insulin treatment
KW - Temperature kinetics
UR - http://www.scopus.com/inward/record.url?scp=38949104056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38949104056&partnerID=8YFLogxK
U2 - 10.1007/s11064-007-9447-1
DO - 10.1007/s11064-007-9447-1
M3 - Article
C2 - 17721819
AN - SCOPUS:38949104056
SN - 0364-3190
VL - 33
SP - 422
EP - 429
JO - Neurochemical Research
JF - Neurochemical Research
IS - 3
ER -
