Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi

Anna C. Renier; Jennifer K. Morrow; Amy J. Graves; Carrie J. Finno; Daniel K. Howe; Sean D. Owens; Eva Tamez-Trevino; Andrea E. Packham; Patricia A. Conrad; Nicola Pusterla

doi:10.1016/j.jevs.2015.10.010

Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi

Anna C. Renier, Jennifer K. Morrow, Amy J. Graves, Carrie J. Finno, Daniel K. Howe, Sean D. Owens, Eva Tamez-Trevino, Andrea E. Packham, Patricia A. Conrad, Nicola Pusterla

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The aim of this study was to compare two serologic tests used to support a diagnosis of equine protozoal myeloencephalitis (EPM). Serum and cerebrospinal fluid (CSF) samples were analyzed for antibodies to Sarcocystis neurona and Neospora hughesi by indirect fluorescent antibody testing (IFAT) and surface antigens of S. neurona and N. hughesi by enzyme-linked immunosorbent assay (ELISA). The samples originated from neurologic horses with confirmed and suspected EPM (nine S. neurona, three N. hughesi), from neurologic horses with confirmed neurologic diseases other than EPM (16 horses) and from healthy horses (10). The IFAT on CSF and ELISA titer ratios showed equal sensitivity in diagnosing EPM caused by S. neurona. The ELISA titer ratios showed slightly greater specificity in diagnosing EPM than the IFAT on CSF. Overall agreement between the IFAT on CSF and ELISA titer ratio was 90.9%. The IFAT on CSF and ELISA serum/CSF ratio are indicated to help support a laboratory diagnosis of EPM.

Original language	English
Pages (from-to)	49-51
Number of pages	3
Journal	Journal of Equine Veterinary Science
Volume	36
DOIs	https://doi.org/10.1016/j.jevs.2015.10.010
State	Published - Jan 1 2016

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Inc.

Keywords

ELISA
EPM
IFAT
Neospora hughesi
Sarcocystis neurona

ASJC Scopus subject areas

Equine

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10.1016/j.jevs.2015.10.010

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Renier, A. C., Morrow, J. K., Graves, A. J., Finno, C. J., Howe, D. K., Owens, S. D., Tamez-Trevino, E., Packham, A. E., Conrad, P. A., & Pusterla, N. (2016). Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi. Journal of Equine Veterinary Science, 36, 49-51. https://doi.org/10.1016/j.jevs.2015.10.010

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title = "Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi",

abstract = "The aim of this study was to compare two serologic tests used to support a diagnosis of equine protozoal myeloencephalitis (EPM). Serum and cerebrospinal fluid (CSF) samples were analyzed for antibodies to Sarcocystis neurona and Neospora hughesi by indirect fluorescent antibody testing (IFAT) and surface antigens of S. neurona and N. hughesi by enzyme-linked immunosorbent assay (ELISA). The samples originated from neurologic horses with confirmed and suspected EPM (nine S. neurona, three N. hughesi), from neurologic horses with confirmed neurologic diseases other than EPM (16 horses) and from healthy horses (10). The IFAT on CSF and ELISA titer ratios showed equal sensitivity in diagnosing EPM caused by S. neurona. The ELISA titer ratios showed slightly greater specificity in diagnosing EPM than the IFAT on CSF. Overall agreement between the IFAT on CSF and ELISA titer ratio was 90.9%. The IFAT on CSF and ELISA serum/CSF ratio are indicated to help support a laboratory diagnosis of EPM.",

keywords = "ELISA, EPM, IFAT, Neospora hughesi, Sarcocystis neurona",

author = "Renier, {Anna C.} and Morrow, {Jennifer K.} and Graves, {Amy J.} and Finno, {Carrie J.} and Howe, {Daniel K.} and Owens, {Sean D.} and Eva Tamez-Trevino and Packham, {Andrea E.} and Conrad, {Patricia A.} and Nicola Pusterla",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

year = "2016",

month = jan,

day = "1",

doi = "10.1016/j.jevs.2015.10.010",

language = "English",

volume = "36",

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Renier, AC, Morrow, JK, Graves, AJ, Finno, CJ, Howe, DK, Owens, SD, Tamez-Trevino, E, Packham, AE, Conrad, PA & Pusterla, N 2016, 'Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi', Journal of Equine Veterinary Science, vol. 36, pp. 49-51. https://doi.org/10.1016/j.jevs.2015.10.010

Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi. / Renier, Anna C.; Morrow, Jennifer K.; Graves, Amy J. et al.
In: Journal of Equine Veterinary Science, Vol. 36, 01.01.2016, p. 49-51.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi

AU - Renier, Anna C.

AU - Morrow, Jennifer K.

AU - Graves, Amy J.

AU - Finno, Carrie J.

AU - Howe, Daniel K.

AU - Owens, Sean D.

AU - Tamez-Trevino, Eva

AU - Packham, Andrea E.

AU - Conrad, Patricia A.

AU - Pusterla, Nicola

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The aim of this study was to compare two serologic tests used to support a diagnosis of equine protozoal myeloencephalitis (EPM). Serum and cerebrospinal fluid (CSF) samples were analyzed for antibodies to Sarcocystis neurona and Neospora hughesi by indirect fluorescent antibody testing (IFAT) and surface antigens of S. neurona and N. hughesi by enzyme-linked immunosorbent assay (ELISA). The samples originated from neurologic horses with confirmed and suspected EPM (nine S. neurona, three N. hughesi), from neurologic horses with confirmed neurologic diseases other than EPM (16 horses) and from healthy horses (10). The IFAT on CSF and ELISA titer ratios showed equal sensitivity in diagnosing EPM caused by S. neurona. The ELISA titer ratios showed slightly greater specificity in diagnosing EPM than the IFAT on CSF. Overall agreement between the IFAT on CSF and ELISA titer ratio was 90.9%. The IFAT on CSF and ELISA serum/CSF ratio are indicated to help support a laboratory diagnosis of EPM.

AB - The aim of this study was to compare two serologic tests used to support a diagnosis of equine protozoal myeloencephalitis (EPM). Serum and cerebrospinal fluid (CSF) samples were analyzed for antibodies to Sarcocystis neurona and Neospora hughesi by indirect fluorescent antibody testing (IFAT) and surface antigens of S. neurona and N. hughesi by enzyme-linked immunosorbent assay (ELISA). The samples originated from neurologic horses with confirmed and suspected EPM (nine S. neurona, three N. hughesi), from neurologic horses with confirmed neurologic diseases other than EPM (16 horses) and from healthy horses (10). The IFAT on CSF and ELISA titer ratios showed equal sensitivity in diagnosing EPM caused by S. neurona. The ELISA titer ratios showed slightly greater specificity in diagnosing EPM than the IFAT on CSF. Overall agreement between the IFAT on CSF and ELISA titer ratio was 90.9%. The IFAT on CSF and ELISA serum/CSF ratio are indicated to help support a laboratory diagnosis of EPM.

KW - ELISA

KW - EPM

KW - IFAT

KW - Neospora hughesi

KW - Sarcocystis neurona

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U2 - 10.1016/j.jevs.2015.10.010

DO - 10.1016/j.jevs.2015.10.010

M3 - Article

AN - SCOPUS:84951749799

SN - 0737-0806

VL - 36

SP - 49

EP - 51

JO - Journal of Equine Veterinary Science

JF - Journal of Equine Veterinary Science

ER -

Diagnosis of Equine Protozoal Myeloencephalitis Using Indirect Fluorescent Antibody Testing and Enzyme-Linked Immunosorbent Assay Titer Ratios for Sarcocystis neurona and Neospora hughesi

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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