Diapause disruption with tebufenozide for early-instar control of the spruce budworm, Choristoneura fumiferana

Daniel Doucet, Caroline Frisco, Michel Cusson, Éric Bauce, Subba Reddy Palli, Bill Tomkins, Basil Arif, Arthur Retnakaran

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


In North America, the eastern spruce budworm, Choristoneura fumiferana Clem., is an important coniferous pest against which tebufenozide has proven effective as a control product. By acting as an ecdysone agonist, tebufenozide can induce precocious moulting in late (fifth-sixth) instars but can also be carried over to the next generation owing to its persistence on foliage. The authors conducted laboratory experiments on first-instar larvae treated with tebufenozide dissolved in acetone. Larvae exposed to doses equal to or above 0.1 μg cm-2 displayed precocious moulting in the second instar after hibernaculum spinning, which effectively disrupted diapause. Larger doses induced moulting in first instars. Evidence is provided that this dose-response difference is related to whether or not an effective dose of tebufenozide is ingested by the first instar prior to the peak of moulting hormone (20-hydroxyecdysone) in first instars. Doses ineffective to kill first instars are carried over to the second instar, where they induce a precocious moult. This type of response to tebufenozide is dependent on the presence of a moulting machinery (the EcR-USP receptor complex) that is ready for ecdysone transduction. Interestingly, ecdysone levels are low in second instars, as measured by a radioimmunoassay, which suggests that diapause in spruce budworm is maintained by a suppression of ecdysone production. Thus, diapause disruption by tebufenozide may well provide an alternative control strategy for this important pest.

Original languageEnglish
Pages (from-to)730-736
Number of pages7
JournalPest Management Science
Issue number8
StatePublished - Aug 2007


  • 20-hydroxyecdysone
  • 20E
  • CHR3
  • Choristoneura fumiferana
  • Diapause
  • Eastern spruce budworm
  • Tebufenozide

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science


Dive into the research topics of 'Diapause disruption with tebufenozide for early-instar control of the spruce budworm, Choristoneura fumiferana'. Together they form a unique fingerprint.

Cite this