Dietary crude protein intake influences rates of whole-body protein synthesis in weanling horses

S. L. Tanner; A. L. Wagner; R. N. Digianantonio; P. A. Harris; J. T. Sylvester; K. L. Urschel

doi:10.1016/j.tvjl.2014.06.002

Dietary crude protein intake influences rates of whole-body protein synthesis in weanling horses

S. L. Tanner, A. L. Wagner, R. N. Digianantonio, P. A. Harris, J. T. Sylvester, K. L. Urschel

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The objective of this study was to measure whole-body protein kinetics in weanling horses receiving forage and one of two different concentrates: (1) commercial crude protein (CCP) concentrate, which with the forage provided 4.1 g CP/kg bodyweight (BW)/day (189 mg lysine (Lys)/kg BW/day), and (2) recommended crude protein (RCP) concentrate which, with the same forage, provided 3.1 g CP/kg BW/day (194 mg Lys/kg BW/day). Blood samples were taken to determine the response of plasma amino acid concentrations to half the daily concentrate allocation. The next day, a 2 h-primed, constant infusion of [¹³C]sodium bicarbonate and a 4 h-primed, constant infusion of [1-¹³C]phenylalanine were used with breath and blood sampling to measure breath ¹³CO₂ and blood [¹³C]phenylalanine enrichment. Horses on the CCP diet showed an increase from baseline in plasma isoleucine, leucine, lysine, threonine, valine, alanine, arginine, asparagine, glutamine, ornithine, proline, serine, and tyrosine at 120 min post-feeding. Baseline plasma amino acid concentrations were greater with the CCP diet for histidine, isoleucine, leucine, threonine, valine, asparagine, proline, and serine. Phenylalanine, lysine, and methionine were greater in the plasma of horses receiving the RCP treatment at 0 and 120 min. Phenylalanine intake was standardized between groups; however, horses receiving the RCP diet had greater rates of phenylalanine oxidation (P = 0.02) and lower rates of non-oxidative phenylalanine disposal (P = 0.04). Lower whole-body protein synthesis indicates a limiting amino acid in the RCP diet.

Original language	English
Pages (from-to)	236-243
Number of pages	8
Journal	Veterinary Journal
Volume	202
Issue number	2
DOIs	https://doi.org/10.1016/j.tvjl.2014.06.002
State	Published - Nov 1 2014

Bibliographical note

Funding Information:
The information reported in this paper (13-07-141) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This project was funded by the first WALTHAM-Buckeye Foundation grant. Lyn Ennis contributed technical expertise helping with sampling and sample analyses. Preliminary results of this project were presented as abstracts at the 2011 Equine Science Society meeting and the 2012 American Academy of Veterinary Nutrition Clinical Nutrition and Research Symposium.

Publisher Copyright:
© 2014 Elsevier Ltd.

Keywords

Amino acids
Horse
Phenylalanine kinetics
Protein intake
Protein synthesis

ASJC Scopus subject areas

Animal Science and Zoology
Veterinary (all)

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10.1016/j.tvjl.2014.06.002

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title = "Dietary crude protein intake influences rates of whole-body protein synthesis in weanling horses",

abstract = "The objective of this study was to measure whole-body protein kinetics in weanling horses receiving forage and one of two different concentrates: (1) commercial crude protein (CCP) concentrate, which with the forage provided 4.1 g CP/kg bodyweight (BW)/day (189 mg lysine (Lys)/kg BW/day), and (2) recommended crude protein (RCP) concentrate which, with the same forage, provided 3.1 g CP/kg BW/day (194 mg Lys/kg BW/day). Blood samples were taken to determine the response of plasma amino acid concentrations to half the daily concentrate allocation. The next day, a 2 h-primed, constant infusion of [13C]sodium bicarbonate and a 4 h-primed, constant infusion of [1-13C]phenylalanine were used with breath and blood sampling to measure breath 13CO2 and blood [13C]phenylalanine enrichment. Horses on the CCP diet showed an increase from baseline in plasma isoleucine, leucine, lysine, threonine, valine, alanine, arginine, asparagine, glutamine, ornithine, proline, serine, and tyrosine at 120 min post-feeding. Baseline plasma amino acid concentrations were greater with the CCP diet for histidine, isoleucine, leucine, threonine, valine, asparagine, proline, and serine. Phenylalanine, lysine, and methionine were greater in the plasma of horses receiving the RCP treatment at 0 and 120 min. Phenylalanine intake was standardized between groups; however, horses receiving the RCP diet had greater rates of phenylalanine oxidation (P = 0.02) and lower rates of non-oxidative phenylalanine disposal (P = 0.04). Lower whole-body protein synthesis indicates a limiting amino acid in the RCP diet.",

keywords = "Amino acids, Horse, Phenylalanine kinetics, Protein intake, Protein synthesis",

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note = "Funding Information: The information reported in this paper (13-07-141) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This project was funded by the first WALTHAM-Buckeye Foundation grant. Lyn Ennis contributed technical expertise helping with sampling and sample analyses. Preliminary results of this project were presented as abstracts at the 2011 Equine Science Society meeting and the 2012 American Academy of Veterinary Nutrition Clinical Nutrition and Research Symposium. Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd.",

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AU - Harris, P. A.

AU - Sylvester, J. T.

AU - Urschel, K. L.

N1 - Funding Information: The information reported in this paper (13-07-141) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This project was funded by the first WALTHAM-Buckeye Foundation grant. Lyn Ennis contributed technical expertise helping with sampling and sample analyses. Preliminary results of this project were presented as abstracts at the 2011 Equine Science Society meeting and the 2012 American Academy of Veterinary Nutrition Clinical Nutrition and Research Symposium. Publisher Copyright: © 2014 Elsevier Ltd.

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Dietary crude protein intake influences rates of whole-body protein synthesis in weanling horses

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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