TY - JOUR
T1 - Dietary ractopamine influences sarcoplasmic proteome profile of pork Longissimus thoracis
AU - Costa-Lima, Bruno R.C.
AU - Suman, Surendranath P.
AU - Li, Shuting
AU - Beach, Carol M.
AU - Silva, Teofilo J.P.
AU - Silveira, Expedito T.F.
AU - Bohrer, Benjamin M.
AU - Boler, Dustin D.
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Dietary ractopamine improves pork leanness, whereas its effect on sarcoplasmic proteome has not been characterized. Therefore, the influence of ractopamine on sarcoplasmic proteome of post-mortem pork Longissimus thoracis muscle was examined. Longissimus thoracis samples were collected from carcasses (24. h post-mortem) of purebred Berkshire barrows (n = 9) managed in mixed-sex pens and fed finishing diets containing ractopamine (RAC; 7.4. mg/kg for 14. days followed by 10.0. mg/kg for 14. days) or without ractopamine for 28. days (CON). Sarcoplasmic proteome was analyzed using two-dimensional electrophoresis and mass spectrometry. Nine protein spots were differentially abundant between RAC and CON groups. Glyceraldehyde-3-phosphate dehydrogenase and phosphoglucomutase-1 were over-abundant in CON, whereas serum albumin, carbonic anhydrase 3, l-lactate dehydrogenase A chain, fructose-bisphosphate aldolase A, and myosin light chain 1/3 were over-abundant in RAC. These results suggest that ractopamine influences the abundance of enzymes involved in glycolytic metabolism, and the differential abundance of glycolytic enzymes could potentially influence the conversion of muscle to meat.
AB - Dietary ractopamine improves pork leanness, whereas its effect on sarcoplasmic proteome has not been characterized. Therefore, the influence of ractopamine on sarcoplasmic proteome of post-mortem pork Longissimus thoracis muscle was examined. Longissimus thoracis samples were collected from carcasses (24. h post-mortem) of purebred Berkshire barrows (n = 9) managed in mixed-sex pens and fed finishing diets containing ractopamine (RAC; 7.4. mg/kg for 14. days followed by 10.0. mg/kg for 14. days) or without ractopamine for 28. days (CON). Sarcoplasmic proteome was analyzed using two-dimensional electrophoresis and mass spectrometry. Nine protein spots were differentially abundant between RAC and CON groups. Glyceraldehyde-3-phosphate dehydrogenase and phosphoglucomutase-1 were over-abundant in CON, whereas serum albumin, carbonic anhydrase 3, l-lactate dehydrogenase A chain, fructose-bisphosphate aldolase A, and myosin light chain 1/3 were over-abundant in RAC. These results suggest that ractopamine influences the abundance of enzymes involved in glycolytic metabolism, and the differential abundance of glycolytic enzymes could potentially influence the conversion of muscle to meat.
KW - Glycolysis
KW - Longissimus thoracis
KW - Ractopamine
KW - Sarcoplasmic proteome
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U2 - 10.1016/j.meatsci.2014.12.008
DO - 10.1016/j.meatsci.2014.12.008
M3 - Article
C2 - 25576742
AN - SCOPUS:84920913974
SN - 0309-1740
VL - 103
SP - 7
EP - 12
JO - Meat Science
JF - Meat Science
ER -