Differences in the kinetics of T cell accumulations in C3H/HeN (Bcg-resistant) and C57BL/6 (Bcg-susceptible) mice infected with Mycobacterium paratuberculosis

Ronald S. Veazey, David W. Horohov, James L. Krahenbuhl, H. Wayne Taylor, Julian L. Oliver, Theron G. Snider

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5 Scopus citations


The accumulation of various T cell subsets in Bcg-susceptible (C57BL/6) and -resistant (C3H/HeN) strains of mice were compared following an intraperitoneal infection with Mycobacterium paratuberculosis. Groups of mice from both strains were killed at 3, 5, 10, 15, 30, and 150 days after infection and lymphocytes were harvested from the peritoneal exudate cells (PEG), spleen, intestinal epithelial lymphocytes (IEL), lamina propria lymphocytes (LPL), Peyer's patches, and mesenteric lymph node (MLN) and labelled with monoclonal antibodies to CD3, CD4, CD8, γδ TCR, CD25, and CD44 for flow cytometric analysis. Uninfected C3H/HeN mice had higher proportions of CD4+ cells in the spleen, MLN, LPL, IEL and Peyer's patches, while uninfected C57BL/6 mice had higher proportions of CD8+ and/or γδ T cells. Significant increases in accumulation of CD8+ and γδ T cells were detected in the peritoneum and other tissues in both strains of mice after infection. Higher CD4/CD8 ratios were observed in most lymphoid tissues of C3H/HeN mice, while increased proportions of CD8+ and/or γδ T cells were present in C57BL/6 mice. These results indicate that significant differences in T cell profiles exist between these two strains of mice, both inherently and in response to infection with M. paratuberculosis. Innately lower levels of CD4+ cells and/or higher percentages of CD8+ and γδ T cells may play a role in the increased susceptibility of C57BL/6 mice to infection with M. paratuberculosis.

Original languageEnglish
Pages (from-to)289-304
Number of pages16
JournalComparative Immunology, Microbiology and Infectious Diseases
Issue number4
StatePublished - Sep 1996

Bibliographical note

Funding Information:
the operation of the flow cytometer and data analysis. We also wish to thank Mae Lopez, Cheryl Crowder, Cindy Berry, J. P. Pasqua, and Julie Loesch for their excellent technical assistance, and Thomas and Christine Lemarchand for their assistance in preparation of the abstract. This manuscript fulfils, in part, the requirements for the degree of Doctor of Philosophy in the Interdepartmental Program in Veterinary Medical Sciences, Department of Veterinary Pathology, Louisiana State University. This research was supported by a USDA Animal Health Funds Grant, LAV # 1651.


  • Bcg gene
  • Mice
  • Mycobacteria
  • Mycobacterium paratuberculosis
  • Nramp gene
  • T lymphocyte

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • General Veterinary
  • Infectious Diseases


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