Abstract
The effects of environmental and handling stress during catfish (Ictalurus punctatus) aquaculture were evaluated to identify the biochemical alterations they induce in the muscle proteome and their impacts on fillet quality. Temperature (25 °C and 33 °C) and oxygen (~ 2.5 mg/L [L] and > 5 mg/L [H]) were manipulated followed by sequential socking (S) and transport (T) stress to evaluate changes in quality when fish were subjected to handling (25-H-ST; temperature-oxygen-handling), oxygen stress (25-L-ST), temperature stress (33-H-ST) and severe stress (33-L-ST). Instrumental color and texture of fillets were evaluated, and muscle proteome profile was analyzed. Fillet redness, yellowness and chroma decreased, and hue angle increased in all treatments except temperature stress (33-H-ST). Alterations in texture compared to controls were observed when oxygen levels were held high. In general, changes in the abundance of structural proteins and those involved in protein regulation and energy metabolism were identified. Rearing under hypoxic conditions demonstrated a shift in metabolism to ketogenic pathways and a suppression of the stress-induced changes as the severity of the stress increased. Increased proteolytic activity observed through the down-regulation of various structural proteins could be responsible for the alterations in color and texture.
| Original language | English |
|---|---|
| Pages (from-to) | 10-18 |
| Number of pages | 9 |
| Journal | Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics |
| Volume | 20 |
| DOIs | |
| State | Published - Dec 1 2016 |
Bibliographical note
Publisher Copyright:© 2016
Funding
We gratefully acknowledge Dr. Jimmy Avery and Dr. Taejo Kim for their guidance in developing the study parameters. We also thank students and technicians: Jordan Embry, Zach Mitchell, Shelby Mathieu and Tyler Sanders that helped with the rearing and husbandry of the fish and those members of the Muscle Foods Processing, Sensory, & Chemistry Laboratory at Mississippi State University for their help in fish processing and data collection We thank the United States Department of Agriculture for use of the aquaculture facilities, Mack Fondren for his help with equipment maintenance and supplies and Dr. Kurt Gamperl for use of equipment. We thank Monil Desai for his help and guidance with protein extraction and separation procedures and Dr. Carol Beach for protein identification. The mass spectrometric analysis was performed at the University of Kentucky's Center for Structural Biology Protein Core Facility, supported in part by funds from the National Institute of Health's National Center for Research Resources Grant P20 RR020171 . Approved for publication as Journal Article Number J-12691 of the Mississippi Agricultural and Forestry Experiment Station under project MIS-326050.
| Funders | Funder number |
|---|---|
| National Institutes of Health (NIH) | P20 RR020171 |
Keywords
- Color
- Environmental stress
- Handling stress
- Proteomics
- Texture
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Aquatic Science
- Animal Science and Zoology
- Molecular Biology
- Genetics