Differential effects of post-implantation time on potassium- versus D-amphetamine-evoked dopamine overflow in the striatum of F344 rats

Matthew S. Purdom, John A. Stanford, Greg A. Gerhardt

Research output: Contribution to journalArticlepeer-review

Abstract

Effects of post-implantation time on potassium (K+)- versus D-amphetamine (D-AMPH)-evoked striatal dopamine (DA) overflow were measured using microdialysis in freely moving young and aged Fischer 344 rats. In one group, samples were collected on the day of probe insertion (Day 1 group). In a second group, samples were collected 24 h after probe insertion (Day 2 group). While analyses revealed no significant differences between the two age groups, the 100 mM K+ stimulus evoked a significantly greater amount of DA overflow in the Day 1 group compared to the Day 2 group. The decrease in 3,4-dihydroxyphenylacetic acid (DOPAC) produced by K+ stimulation was not influenced by post-implantation time. The effect of the 250 μM D-AMPH stimulus on DA overflow did not differ between the Day 1 and Day 2 groups, nor did the decrease in DOPAC that accompanied D-AMPH stimulation. These results support the hypothesis that under some stimulus conditions, post-implantation time is an important variable in microdialysis studies.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalNeuroscience Letters
Volume348
Issue number2
DOIs
StatePublished - Sep 11 2003

Bibliographical note

Funding Information:
This work was supported by grants from USPHS AG06434, AG13494, NS39787, and a level II Research Scientist Award (MH01245) to G. Gerhardt, and a Kentucky Opportunity Fellowship to M. Purdom. The authors would like to thank Peter Huettl for expert assistance with HPLC-EC.

Keywords

  • Aging
  • Basal ganglia
  • Dopamine
  • Movement
  • Nigrostriatal

ASJC Scopus subject areas

  • General Neuroscience

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