TY - JOUR
T1 - Differential effects of T-cell activation on gastric and small bowel permeability in alcohol-consuming mice
AU - Alnadjim, Ziad
AU - Kayali, Zeid
AU - Haddad, Wael
AU - Holmes, Earl W.
AU - Keshavarzian, Ali
AU - Mittal, Navdha
AU - Ivancic, David
AU - Koehler, Ronald
AU - Goldsmith, David
AU - Waltenbaugh, Carl
AU - Barrett, Terrence A.
PY - 2002/9
Y1 - 2002/9
N2 - Background: A number of variables influence the effect(s) of alcohol on distinct segments of the intestine. In these studies, we examined the effect of T-cell activation on gastric and small bowel permeability in alcohol-fed mice. Methods: Gastric permeability was assessed using sucrose absorption, whereas small bowel permeability was followed using the ratio of lactulose to mannitol absorption and inulin absorption. T cells were activated by injecting antigen OVA323-339 into DO11.10 T-cell receptor transgenic mice. Results: T-cell activation increased gastric and small bowel permeability through a pathway mediated by interferon-γ and tumor necrosis factor. In mice that were fed a liquid diet that contained 30% ethanol-derived calories for 2 weeks, T-cell activation increased gastric permeability to levels greater than that observed in solid diet or pair-fed, liquid control diet. By comparison, changes in small bowel permeability induced by T-cell activation were abrogated in alcohol-fed mice. Analysis of intestinal cytokine mRNA levels (interferon-γ and tumor necrosis factor) indicated that relevant mucosal T-cell function was preserved in alcohol-fed mice Conclusions: Overall, these data suggest that alcohol potentiates the effects of T-cell activation on gastric permeability, at the same time blunting effects on small bowel permeability.
AB - Background: A number of variables influence the effect(s) of alcohol on distinct segments of the intestine. In these studies, we examined the effect of T-cell activation on gastric and small bowel permeability in alcohol-fed mice. Methods: Gastric permeability was assessed using sucrose absorption, whereas small bowel permeability was followed using the ratio of lactulose to mannitol absorption and inulin absorption. T cells were activated by injecting antigen OVA323-339 into DO11.10 T-cell receptor transgenic mice. Results: T-cell activation increased gastric and small bowel permeability through a pathway mediated by interferon-γ and tumor necrosis factor. In mice that were fed a liquid diet that contained 30% ethanol-derived calories for 2 weeks, T-cell activation increased gastric permeability to levels greater than that observed in solid diet or pair-fed, liquid control diet. By comparison, changes in small bowel permeability induced by T-cell activation were abrogated in alcohol-fed mice. Analysis of intestinal cytokine mRNA levels (interferon-γ and tumor necrosis factor) indicated that relevant mucosal T-cell function was preserved in alcohol-fed mice Conclusions: Overall, these data suggest that alcohol potentiates the effects of T-cell activation on gastric permeability, at the same time blunting effects on small bowel permeability.
KW - Alcohol
KW - Gastric Permeability
KW - Mouse
KW - T-Cell Activation
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U2 - 10.1097/00000374-200209000-00018
DO - 10.1097/00000374-200209000-00018
M3 - Article
C2 - 12351940
AN - SCOPUS:18544385811
SN - 0145-6008
VL - 26
SP - 1436
EP - 1443
JO - Alcoholism: Clinical and Experimental Research
JF - Alcoholism: Clinical and Experimental Research
IS - 9
ER -