Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells

Tissa T. Manavalan; Yun Teng; Savitri N. Appana; Susmita Datta; Theodore S. Kalbfleisch; Yong Li; Carolyn M. Klinge

doi:10.1016/j.canlet.2011.08.018

Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells

Tissa T. Manavalan, Yun Teng, Savitri N. Appana, Susmita Datta, Theodore S. Kalbfleisch, Yong Li, Carolyn M. Klinge

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

Microarrays identified miRNAs differentially expressed and 4-hydroxytamoxifen (4-OHT) regulated in MCF-7 endocrine-sensitive versus resistant LY2 human breast cancer cells. 97 miRNAs were differentially expressed in MCF-7 versus LY2 cells. Opposite expression of miRs-10a, 21, 22, 29a, 93, 125b, 181, 200a, 200b, 200c, 205, and 222 was confirmed. Bioinformatic analyses to impute the biological significance of these miRNAs identified 36 predicted gene targets from those regulated by 4-OHT in MCF-7 cells. Agreement in the direction of anticipated regulation was detected for 12 putative targets. These miRNAs with opposite expression between the two cell lines may be involved in endocrine resistance.

Original language	English
Pages (from-to)	26-43
Number of pages	18
Journal	Cancer Letters
Volume	313
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2011.08.018
State	Published - Dec 26 2011

Bibliographical note

Funding Information:
This work was supported by NIH R21 CA124811 and NIH R01 CA138410 to CMK and in part by P30ES01443 for Bioinformatic analysis by TSK. We thank Dr. Nalinie S. Wickramasinghe for performing the cell treatments and isolating the RNA sent to Exiqon. We thank Dr. Barbara J. Clark for her review of our manuscript.

Keywords

Breast cancer
Endocrine-resistance
MicroRNA
SERMs
Tamoxifen-resistance

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.canlet.2011.08.018

Cite this

@article{ef430a7b4cb54da184922e62765b8e24,

title = "Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells",

abstract = "Microarrays identified miRNAs differentially expressed and 4-hydroxytamoxifen (4-OHT) regulated in MCF-7 endocrine-sensitive versus resistant LY2 human breast cancer cells. 97 miRNAs were differentially expressed in MCF-7 versus LY2 cells. Opposite expression of miRs-10a, 21, 22, 29a, 93, 125b, 181, 200a, 200b, 200c, 205, and 222 was confirmed. Bioinformatic analyses to impute the biological significance of these miRNAs identified 36 predicted gene targets from those regulated by 4-OHT in MCF-7 cells. Agreement in the direction of anticipated regulation was detected for 12 putative targets. These miRNAs with opposite expression between the two cell lines may be involved in endocrine resistance.",

keywords = "Breast cancer, Endocrine-resistance, MicroRNA, SERMs, Tamoxifen-resistance",

author = "Manavalan, {Tissa T.} and Yun Teng and Appana, {Savitri N.} and Susmita Datta and Kalbfleisch, {Theodore S.} and Yong Li and Klinge, {Carolyn M.}",

note = "Funding Information: This work was supported by NIH R21 CA124811 and NIH R01 CA138410 to CMK and in part by P30ES01443 for Bioinformatic analysis by TSK. We thank Dr. Nalinie S. Wickramasinghe for performing the cell treatments and isolating the RNA sent to Exiqon. We thank Dr. Barbara J. Clark for her review of our manuscript. ",

year = "2011",

month = dec,

day = "26",

doi = "10.1016/j.canlet.2011.08.018",

language = "English",

volume = "313",

pages = "26--43",

number = "1",

}

TY - JOUR

T1 - Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells

AU - Manavalan, Tissa T.

AU - Teng, Yun

AU - Appana, Savitri N.

AU - Datta, Susmita

AU - Kalbfleisch, Theodore S.

AU - Li, Yong

AU - Klinge, Carolyn M.

N1 - Funding Information: This work was supported by NIH R21 CA124811 and NIH R01 CA138410 to CMK and in part by P30ES01443 for Bioinformatic analysis by TSK. We thank Dr. Nalinie S. Wickramasinghe for performing the cell treatments and isolating the RNA sent to Exiqon. We thank Dr. Barbara J. Clark for her review of our manuscript.

PY - 2011/12/26

Y1 - 2011/12/26

N2 - Microarrays identified miRNAs differentially expressed and 4-hydroxytamoxifen (4-OHT) regulated in MCF-7 endocrine-sensitive versus resistant LY2 human breast cancer cells. 97 miRNAs were differentially expressed in MCF-7 versus LY2 cells. Opposite expression of miRs-10a, 21, 22, 29a, 93, 125b, 181, 200a, 200b, 200c, 205, and 222 was confirmed. Bioinformatic analyses to impute the biological significance of these miRNAs identified 36 predicted gene targets from those regulated by 4-OHT in MCF-7 cells. Agreement in the direction of anticipated regulation was detected for 12 putative targets. These miRNAs with opposite expression between the two cell lines may be involved in endocrine resistance.

AB - Microarrays identified miRNAs differentially expressed and 4-hydroxytamoxifen (4-OHT) regulated in MCF-7 endocrine-sensitive versus resistant LY2 human breast cancer cells. 97 miRNAs were differentially expressed in MCF-7 versus LY2 cells. Opposite expression of miRs-10a, 21, 22, 29a, 93, 125b, 181, 200a, 200b, 200c, 205, and 222 was confirmed. Bioinformatic analyses to impute the biological significance of these miRNAs identified 36 predicted gene targets from those regulated by 4-OHT in MCF-7 cells. Agreement in the direction of anticipated regulation was detected for 12 putative targets. These miRNAs with opposite expression between the two cell lines may be involved in endocrine resistance.

KW - Breast cancer

KW - Endocrine-resistance

KW - MicroRNA

KW - SERMs

KW - Tamoxifen-resistance

UR - http://www.scopus.com/inward/record.url?scp=80054090363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054090363&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2011.08.018

DO - 10.1016/j.canlet.2011.08.018

M3 - Article

C2 - 21955614

AN - SCOPUS:80054090363

SN - 0304-3835

VL - 313

SP - 26

EP - 43

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this