Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells

Tissa T. Manavalan, Yun Teng, Savitri N. Appana, Susmita Datta, Theodore S. Kalbfleisch, Yong Li, Carolyn M. Klinge

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

Microarrays identified miRNAs differentially expressed and 4-hydroxytamoxifen (4-OHT) regulated in MCF-7 endocrine-sensitive versus resistant LY2 human breast cancer cells. 97 miRNAs were differentially expressed in MCF-7 versus LY2 cells. Opposite expression of miRs-10a, 21, 22, 29a, 93, 125b, 181, 200a, 200b, 200c, 205, and 222 was confirmed. Bioinformatic analyses to impute the biological significance of these miRNAs identified 36 predicted gene targets from those regulated by 4-OHT in MCF-7 cells. Agreement in the direction of anticipated regulation was detected for 12 putative targets. These miRNAs with opposite expression between the two cell lines may be involved in endocrine resistance.

Original languageEnglish
Pages (from-to)26-43
Number of pages18
JournalCancer Letters
Volume313
Issue number1
DOIs
StatePublished - Dec 26 2011

Bibliographical note

Funding Information:
This work was supported by NIH R21 CA124811 and NIH R01 CA138410 to CMK and in part by P30ES01443 for Bioinformatic analysis by TSK. We thank Dr. Nalinie S. Wickramasinghe for performing the cell treatments and isolating the RNA sent to Exiqon. We thank Dr. Barbara J. Clark for her review of our manuscript.

Keywords

  • Breast cancer
  • Endocrine-resistance
  • MicroRNA
  • SERMs
  • Tamoxifen-resistance

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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