Abstract
Microarrays identified miRNAs differentially expressed and 4-hydroxytamoxifen (4-OHT) regulated in MCF-7 endocrine-sensitive versus resistant LY2 human breast cancer cells. 97 miRNAs were differentially expressed in MCF-7 versus LY2 cells. Opposite expression of miRs-10a, 21, 22, 29a, 93, 125b, 181, 200a, 200b, 200c, 205, and 222 was confirmed. Bioinformatic analyses to impute the biological significance of these miRNAs identified 36 predicted gene targets from those regulated by 4-OHT in MCF-7 cells. Agreement in the direction of anticipated regulation was detected for 12 putative targets. These miRNAs with opposite expression between the two cell lines may be involved in endocrine resistance.
| Original language | English |
|---|---|
| Pages (from-to) | 26-43 |
| Number of pages | 18 |
| Journal | Cancer Letters |
| Volume | 313 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 26 2011 |
Bibliographical note
Funding Information:This work was supported by NIH R21 CA124811 and NIH R01 CA138410 to CMK and in part by P30ES01443 for Bioinformatic analysis by TSK. We thank Dr. Nalinie S. Wickramasinghe for performing the cell treatments and isolating the RNA sent to Exiqon. We thank Dr. Barbara J. Clark for her review of our manuscript.
Funding
This work was supported by NIH R21 CA124811 and NIH R01 CA138410 to CMK and in part by P30ES01443 for Bioinformatic analysis by TSK. We thank Dr. Nalinie S. Wickramasinghe for performing the cell treatments and isolating the RNA sent to Exiqon. We thank Dr. Barbara J. Clark for her review of our manuscript.
| Funders | Funder number |
|---|---|
| TSK | |
| National Institutes of Health (NIH) | R01 CA138410, P30ES01443 |
| National Childhood Cancer Registry – National Cancer Institute | R21CA124811 |
Keywords
- Breast cancer
- Endocrine-resistance
- MicroRNA
- SERMs
- Tamoxifen-resistance
ASJC Scopus subject areas
- Oncology
- Cancer Research
