Differential induction of sesquiterpene metabolism in tobacco cell suspension cultures by methyl jasmonate and fungal elicitor

Alejandra Mandujano-Chávez, Mark A. Schoenbeck, Lyle F. Ralston, Edmundo Lozoya-Gloria, Joseph Chappell

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Jasmonates are well documented for their ability to modulate the expression of plant genes and to influence specific aspects of disease/pest resistance traits. We and others have been studying the synthesis of sesquiterpene phytoalexins in elicitor/pathogen-challenged plants and have sought to determine if methyl jasmonate (MeJA) could substitute for fungal elicitors in the induction of capsidiol accumulation by tobacco cell cultures. The current results demonstrate that MeJA does in fact induce phytoalexin accumulation, but with a much more delayed induction time course than elicitor. While elicitor treatment induced strong but transient changes in key enzymes of sesquiterpene biosynthesis, sesquiterpene cyclase, and aristolochene/deoxy-capsidiol hydroxylase, MeJA did not. Instead, MeJA caused a protracted induction of cyclase activity and only a low level of hydroxylase activity. MeJA induced the expression of at least two sesquiterpene cyclase genes, including one that had not been observed previously in elicitor-induced mRNA populations. Only a small portion of the total sesquiterpene cyclase mRNA induced by MeJA was associated with polysomal RNA, suggesting that the MeJA treatment imposed both transcriptional and posttranscriptional regulation in tobacco cells. These results are not consistent with MeJA playing a role in orchestrating defense responses in elicitor-treated tobacco cells, but do provide evidence that MeJA induces a subset of genes coding for the biosynthesis of sesquiterpene phytoalexins.

Original languageEnglish
Pages (from-to)285-294
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume381
Issue number2
DOIs
StatePublished - Sep 15 2000

Bibliographical note

Funding Information:
This work was supported by a grant from the National Science Foundation and the Kentucky Agricultural Experiment Station. A.M.C. is recipient of a Doctoral fellowship (No. 96382) from CONACyT (México).

Keywords

  • Methyl jasmonate-defense response
  • Phytoalexins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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