Differential localization of IL-2- and -15 receptor chains in membrane rafts of human T cells

Jens Goebel, Kathy Forrest, Lorri Morford, Thomas L. Roszman

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


We studied whether cytokine receptors (Rs) on T cells associate with lipid microdomains ("rafts"). Low-dose phytohemagglutinin (PHA)-stimulated human T cells were separated into cytoplasmic, membrane, and raft fractions by buoyant density centrifugation. Examination of these fractions for the presence of interleukin (IL)-2- and -15R chains and associated signaling molecules by Western blotting revealed marked, selective enrichment of the IL-2/15R β-chain in rafts before IL-2 stimulation. After IL-2 stimulation, a substantial amount of the β-chain was found in the membrane fraction. This partial translocation was also observed for the β-chain-associated molecules JAK-1, p56 lck, and grb-2. Finally, raft disruption with methyl-β-cyclodextrin (MBCD) attenuated IL-2-induced tyrosine phosphorylation events and selectively decreased the surface expression of the IL-2/15R β-chain detected by flow cytometry. These results show that the IL-2/15R β-chain is enriched in rafts obtained from low-dose, PHA-stimulated T cells, that IL-2 binding alters this enrichment, and that this enrichment may be functionally relevant as a possible mechanism to ensure cytokine selectivity and specificity.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalJournal of Leukocyte Biology
Issue number1
StatePublished - Jul 1 2002


  • Cytokine receptors
  • Signal transduction
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology


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