Differential nuclease sensitivity identifies tight contacts between yeast pre-mRNA and spliceosomes.

B. C. Rymond, M. Rosbash

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The oligonucleotide-directed RNase H sensitivity of a yeast (Saccharomyces cerevisiae) pre-mRNA was determined in an in vitro splicing reaction. While most of the pre-mRNA was sensitive to cleavage, the regions of the 5' splice site and TACTAAC box were found to be highly resistant. The biochemical requirements for protection against nuclease attack parallel those of both spliceosome formation and splicing. Most of the uncleaved pre-mRNA remaining after RNase H challenge was found associated with two forms of the yeast spliceosome. Differences in the RNase H sensitivity of pre-mRNA found in the two spliceosome forms indicate an increased association of splicing factors with the 5' splice site during spliceosome assembly.

Original languageEnglish
Pages (from-to)3517-3523
Number of pages7
JournalEMBO Journal
Volume5
Issue number13
DOIs
StatePublished - Dec 20 1986

ASJC Scopus subject areas

  • Neuroscience (all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (all)
  • Immunology and Microbiology (all)

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