Differential ototoxicities induced by lead acetate and tetraethyl lead1

Umit Tuncel, William J. Clerici, Raleigh O. Jones

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Lead poisoning disrupts many biological structures and functions, including those of the auditory system. This study examined the ototoxic effects of lead acetate (LA) and tetraethyl lead (TEL) of equal lead content on cochlear function and the ability of α-phenyl-tert-butyl-nitrone (PBN) to attenuate such effects. Baseline 1.0 μV cochlear microphonic (CM) and compound action potential (CAP) responses were recorded and animals administered either PBN (100 mg/kg, i.p.) or an equal volume of 0.9% saline, followed by an i.p. injection of LA (50 mg/kg) in an ethanol vehicle, TEL (42.7 mg/kg) in a corn oil vehicle, corn oil or ethanol vehicle alone. Two hours after administration, post-exposure CM and CAP responses were recorded. CAP threshold shifts in the saline-LA group were elevated by 5-10 dB at mid to high frequencies relative to controls (20-24 kHz, P < 0.05). Mean CAP threshold shifts in the saline-TEL were significantly greater than those of both control groups at all tested frequencies except 2 kHz (P < 0.001). However, threshold shifts in the group receiving PBN prior to TEL were significantly smaller than shifts in the group receiving saline prior to TEL (P < 0.01). These data suggest that TEL is more ototoxic than is LA and that free radicals partially mediate TEL-induced CAP disruption.

Original languageEnglish
Pages (from-to)113-123
Number of pages11
JournalHearing Research
Volume166
Issue number1-2
DOIs
StatePublished - 2002

Bibliographical note

Funding Information:
The authors thank Dr. William Pierce of the Department of Pharmacology and Toxicology, University of Louisville for determination of blood lead levels. This project was partially supported by Grant DC06701 from the National Institute of Environmental Health Sciences (NIEHS), NIH. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIEHS, NIH.

Keywords

  • Cochlear microphonic
  • Compound action potential
  • Lead compounds
  • Ototoxicity
  • Reactive oxygen species
  • α-Phenyl-tert-butyl-nitrone

ASJC Scopus subject areas

  • Sensory Systems

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