Differential pH sensitivity of tissue superoxide dismutases

Samir P. Patel; Surendra S. Katyare

doi:10.1007/BF02912927

Differential pH sensitivity of tissue superoxide dismutases

Samir P. Patel, Surendra S. Katyare

Physiology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Superoxide dismutase (SOD) activities in the human and rat RBCs and rat liver, kidney, brain and heart mitochondria as well as cytosolic fractions were determined by the pyrogallol assay procedure with slight modifications. Measurements were carried out in 0.1 M potassium phosphate buffer pH 8.0 and 9.2 to assess the pH stability of the SODs from various systems. Under these conditions the SODs from different systems including RBCs exhibited differential pH stability i.e. they displayed differential susceptibility at pH 9.2. Even in a given tissue, the mitochondrial and cytosolic SODs were inactivated differentially at pH 9.2. We also observed that the total mitochondrial and cytosolic SOD contents show a tissue-specific pattern. Our results also suggest that measurements carried out at pH 8.0 may give more realistic estimates of SOD activities.

Original language	English
Pages (from-to)	129-133
Number of pages	5
Journal	Indian Journal of Clinical Biochemistry
Volume	21
Issue number	2
DOIs	https://doi.org/10.1007/BF02912927
State	Published - 2006

Keywords

Cytosolic SOD
Erythrocyte SOD
Mitochondrial SOD
Superoxide dismutase
pH sensitivity

ASJC Scopus subject areas

Clinical Biochemistry

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10.1007/BF02912927

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title = "Differential pH sensitivity of tissue superoxide dismutases",

abstract = "Superoxide dismutase (SOD) activities in the human and rat RBCs and rat liver, kidney, brain and heart mitochondria as well as cytosolic fractions were determined by the pyrogallol assay procedure with slight modifications. Measurements were carried out in 0.1 M potassium phosphate buffer pH 8.0 and 9.2 to assess the pH stability of the SODs from various systems. Under these conditions the SODs from different systems including RBCs exhibited differential pH stability i.e. they displayed differential susceptibility at pH 9.2. Even in a given tissue, the mitochondrial and cytosolic SODs were inactivated differentially at pH 9.2. We also observed that the total mitochondrial and cytosolic SOD contents show a tissue-specific pattern. Our results also suggest that measurements carried out at pH 8.0 may give more realistic estimates of SOD activities.",

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AU - Patel, Samir P.

AU - Katyare, Surendra S.

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N2 - Superoxide dismutase (SOD) activities in the human and rat RBCs and rat liver, kidney, brain and heart mitochondria as well as cytosolic fractions were determined by the pyrogallol assay procedure with slight modifications. Measurements were carried out in 0.1 M potassium phosphate buffer pH 8.0 and 9.2 to assess the pH stability of the SODs from various systems. Under these conditions the SODs from different systems including RBCs exhibited differential pH stability i.e. they displayed differential susceptibility at pH 9.2. Even in a given tissue, the mitochondrial and cytosolic SODs were inactivated differentially at pH 9.2. We also observed that the total mitochondrial and cytosolic SOD contents show a tissue-specific pattern. Our results also suggest that measurements carried out at pH 8.0 may give more realistic estimates of SOD activities.

AB - Superoxide dismutase (SOD) activities in the human and rat RBCs and rat liver, kidney, brain and heart mitochondria as well as cytosolic fractions were determined by the pyrogallol assay procedure with slight modifications. Measurements were carried out in 0.1 M potassium phosphate buffer pH 8.0 and 9.2 to assess the pH stability of the SODs from various systems. Under these conditions the SODs from different systems including RBCs exhibited differential pH stability i.e. they displayed differential susceptibility at pH 9.2. Even in a given tissue, the mitochondrial and cytosolic SODs were inactivated differentially at pH 9.2. We also observed that the total mitochondrial and cytosolic SOD contents show a tissue-specific pattern. Our results also suggest that measurements carried out at pH 8.0 may give more realistic estimates of SOD activities.

KW - Cytosolic SOD

KW - Erythrocyte SOD

KW - Mitochondrial SOD

KW - Superoxide dismutase

KW - pH sensitivity

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Differential pH sensitivity of tissue superoxide dismutases

Abstract

Keywords

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